Multi-posttranscriptional regulations lessen the repressive effect of SRPK1 on brown adipogenesis

研究成果: 雜誌貢獻文章同行評審

4 引文 斯高帕斯(Scopus)

摘要

Alternative splicing has been widely demonstrated to function as pivotal regulation in specifying cellular fates and biological functions. The relative expression or cellular localization of a splicing factor constitutes an important mechanism in spatiotemporal programming of cell- and stage-specific splicing profiles. In this study, results of deep RNA-sequencing (RNA-Seq) analyses first revealed the reprogrammed splicing profile and reduced expression of serine/arginine-rich splicing factor protein kinase 1 (SRPK1) throughout the development of brown adipose tissue (BAT). A gradual increase in the exon 10-skipped SRPK1 transcript, a potential target of a nonsense-mediated decay (NMD) mechanism, was noted during brown adipogenesis. Elevated RBM4a constituted the regulatory mechanism that led to skipping of SRPK1 exon 10. Moreover, brown adipogenesis-induced upregulation of microRNA (miR)-485 interfered with SRPK1 expression by targeting its 3′-untranslated region (UTR). Depletion of endogenous SRPK1 enhanced the development of C3H10T1/2 cells toward brown adipocytes. Taking our results together, multiple post-transcriptional regulations reduced SRPK1 expression, which subsequently affected brown adipogenesis.

原文英語
頁(從 - 到)503-514
頁數12
期刊Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
1863
發行號5
DOIs
出版狀態已發佈 - 五月 2018

ASJC Scopus subject areas

  • 分子生物學
  • 細胞生物學

指紋

深入研究「Multi-posttranscriptional regulations lessen the repressive effect of SRPK1 on brown adipogenesis」主題。共同形成了獨特的指紋。

引用此