摘要
The aim of this study was to characterize a nasally delivered bioadhesive liposome using an inactivated H5N3 virus as a model antigen. Bioadhesive liposomes were developed using tremella (T) or xanthan gum (XG) as the bioadhesive polysaccharide. Using chickens as the target animal, we evaluated whether delivery of a bioadhesive liposomal influenza vaccine via a mucosal site of infection could improve vaccine effectiveness. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cytotoxicity assays demonstrated that T, XG and liposomes were non toxic to chicken spleen macrophages. Enzyme-linked immunosorbent assay (ELISA) was used to determine the adjuvant effect of the bioadhesive liposomal-vaccines. Chickens immunized with a low dose (200 μL) of bioadhesive liposomal influenza vaccine had significantly higher mucosal and serum antibody levels (P <0.05). In addition, liposomes mixed with a low-viscosity bioadhesive gel used for nasal delivery resulted in superior antibody responses compared with liposomes mixed with a high-viscosity gel (P <0.05). This suggest that a low-viscosity gel mixed with liposomes is more suitable for nasal delivery, and that chickens elicit higher mucosal secretory immunoglobulin A (s-IgA) and serum IgG after two vaccinations.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 5862-5868 |
| 頁數 | 7 |
| 期刊 | Biomaterials |
| 卷 | 30 |
| 發行號 | 29 |
| DOIs | |
| 出版狀態 | 已發佈 - 十月 2009 |
指紋
ASJC Scopus subject areas
- Biomaterials
- Bioengineering
- Ceramics and Composites
- Mechanics of Materials
- Biophysics
引用此文
Mucoadhesive liposomes for intranasal immunization with an avian influenza virus vaccine in chickens. / Chiou, Chwei Jang; Tseng, Li Ping; Deng, Ming Chung; Jiang, Pei Rong; Tasi, Shang Li; Chung, Tze Wen; Huang, Yi You; Liu, Der Zen.
於: Biomaterials, 卷 30, 編號 29, 10.2009, p. 5862-5868.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Mucoadhesive liposomes for intranasal immunization with an avian influenza virus vaccine in chickens
AU - Chiou, Chwei Jang
AU - Tseng, Li Ping
AU - Deng, Ming Chung
AU - Jiang, Pei Rong
AU - Tasi, Shang Li
AU - Chung, Tze Wen
AU - Huang, Yi You
AU - Liu, Der Zen
PY - 2009/10
Y1 - 2009/10
N2 - The aim of this study was to characterize a nasally delivered bioadhesive liposome using an inactivated H5N3 virus as a model antigen. Bioadhesive liposomes were developed using tremella (T) or xanthan gum (XG) as the bioadhesive polysaccharide. Using chickens as the target animal, we evaluated whether delivery of a bioadhesive liposomal influenza vaccine via a mucosal site of infection could improve vaccine effectiveness. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cytotoxicity assays demonstrated that T, XG and liposomes were non toxic to chicken spleen macrophages. Enzyme-linked immunosorbent assay (ELISA) was used to determine the adjuvant effect of the bioadhesive liposomal-vaccines. Chickens immunized with a low dose (200 μL) of bioadhesive liposomal influenza vaccine had significantly higher mucosal and serum antibody levels (P <0.05). In addition, liposomes mixed with a low-viscosity bioadhesive gel used for nasal delivery resulted in superior antibody responses compared with liposomes mixed with a high-viscosity gel (P <0.05). This suggest that a low-viscosity gel mixed with liposomes is more suitable for nasal delivery, and that chickens elicit higher mucosal secretory immunoglobulin A (s-IgA) and serum IgG after two vaccinations.
AB - The aim of this study was to characterize a nasally delivered bioadhesive liposome using an inactivated H5N3 virus as a model antigen. Bioadhesive liposomes were developed using tremella (T) or xanthan gum (XG) as the bioadhesive polysaccharide. Using chickens as the target animal, we evaluated whether delivery of a bioadhesive liposomal influenza vaccine via a mucosal site of infection could improve vaccine effectiveness. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cytotoxicity assays demonstrated that T, XG and liposomes were non toxic to chicken spleen macrophages. Enzyme-linked immunosorbent assay (ELISA) was used to determine the adjuvant effect of the bioadhesive liposomal-vaccines. Chickens immunized with a low dose (200 μL) of bioadhesive liposomal influenza vaccine had significantly higher mucosal and serum antibody levels (P <0.05). In addition, liposomes mixed with a low-viscosity bioadhesive gel used for nasal delivery resulted in superior antibody responses compared with liposomes mixed with a high-viscosity gel (P <0.05). This suggest that a low-viscosity gel mixed with liposomes is more suitable for nasal delivery, and that chickens elicit higher mucosal secretory immunoglobulin A (s-IgA) and serum IgG after two vaccinations.
KW - Adjuvant
KW - Intranasal
KW - Liposome
KW - Mucoadhesive
KW - Newcastle disease virus
KW - s-IgA
UR - http://www.scopus.com/inward/record.url?scp=68549088703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68549088703&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2009.06.046
DO - 10.1016/j.biomaterials.2009.06.046
M3 - Article
C2 - 19608270
AN - SCOPUS:68549088703
VL - 30
SP - 5862
EP - 5868
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 29
ER -