Motility and protein phosphorylation in healthy and asthenozoospermic sperm

Chying Chyuan Chan, Hao Ai Shui, Ching Hsiang Wu, Chih Yuan Wang, Guang Huan Sun, Han Min Chen, Gwo Jang Wu

研究成果: 雜誌貢獻文章同行評審

58 引文 斯高帕斯(Scopus)

摘要

The majority of male infertility results from poor sperm motility. A direct link between altered protein phosphorylation and aberrant sperm motility has not been established. To address this issue, sperm samples obtained from 20 donors with healthy sperm and 20 donors with aberrantly motile sperm were subjected to computer assisted semen analysis (CASA), proteomic analysis, Western blot, and immunofluorescent staining. Proteomic analysis identified 12 protein spots as having differential phosphorylation, including gamma-tubulin complex associated protein 2 (GCP2). Western blot and immunofluorescence demonstrated differential expression of gamma-tubulin between healthy and aberrantly motile sperm. In conclusion, hypophosphorylated proteins and reduced expression of gamma-tubulin may be associated with low motility sperm.

原文英語
頁(從 - 到)5382-5386
頁數5
期刊Journal of Proteome Research
8
發行號11
DOIs
出版狀態已發佈 - 十一月 6 2009
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 化學 (全部)

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