Monoamine oxidase B and free radical scavenging activities of natural flavonoids in Melastoma candidum D. Don

M. H. Lee, R. D. Lin, L. Y. Shen, Ling-Ling Yang, K. Y. Yen, W. C. Hou

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79 引文 斯高帕斯(Scopus)

摘要

Monoamine oxidase type B (MAO-B) activity and free radicals are elevated in certain neurological diseases. Four natural flavonoids, quercitrin, isoquercitrin, rutin, and quercetin, were isolated for the first time from the leaves of Melastoma candidum D. Don. They exhibited an inhibitory effect on MAO-B. These potent flavonoids were purified using bioassay-guided fractionation and were separated by Diaion, Sephadex LH-20, and MCI CHP20P columns. The IC50 values of the four potent flavonoids, quercitrin, isoquercitrin, rutin, and quercetin on monoamine oxidase were 19.06, 11.64, 3.89, and 10.89 μM and enzyme kinetics analysis revealed apparent inhibition constants (Kj) of 21.01, 2.72, 1.83, and 7.95 μM, respectively, on the substrate, benzylamine. The four potent compounds also exhibited hydroxyl radical scavenging activity as determined using a spin trapping electron spin resonance method. This suggests that the four flavonoids from M. candidum possess both MAO-B inhibitory and free radical scavenging activities. These important properties may be used for preventing some neurodegenerative diseases in the future.

原文英語
頁(從 - 到)5551-5555
頁數5
期刊Journal of Agricultural and Food Chemistry
49
發行號11
DOIs
出版狀態已發佈 - 2001

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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