Monitoring laser-tissue interaction by non-linear optics

Tsung-Han Tsai, Jin Ning Lee, Jung Yi Chan, Jr Ting Hsu, Hsin Yuan Tan, Chen Yuan Dong, Woan-Ruoh Lee, Sung Jan Lin

研究成果: 書貢獻/報告類型會議貢獻

2 引文 斯高帕斯(Scopus)

摘要

To assess laser therapies in clinical practice, histologic examinations were commonly used. But histologic examinations were invasive and not real-time in nature. In this work, we validate multiphoton microscopy as a useful modality in evaluating laser-tissue reaction in vivo. Multiphoton microscopy based on femtosecond titanium-sapphire laser system were used to evaluate autoflurescence (AF) and second harmonic generation (SHG). Nude mouse skin was irradiated with Erbium:YAG laser at low to high fluence. High resolutional images can be obtained by multiphoton microscopy. At low fluence, Erbium:YAG laser can selectively loosen compact stratum corneum with minimal injury to basal layer. At high fluence, ablated keratinocytes and residual debris can be imaged. The laser thermal effect on dermis could be measured by SHG signals of collagen fibers. SHG decreased as laser fluence increased. Multiphoton microscopy is a useful in-vivo technique in evaluating ablative and thermal effects of Erbium:YAG laser on nude mouse skin.
原文英語
主出版物標題Progress in Biomedical Optics and Imaging - Proceedings of SPIE
6842
DOIs
出版狀態已發佈 - 2008
事件Photonic Therapeutics and Diagnostics IV - San Jose, CA, 美国
持續時間: 一月 19 2008一月 19 2008

其他

其他Photonic Therapeutics and Diagnostics IV
國家/地區美国
城市San Jose, CA
期間1/19/081/19/08

ASJC Scopus subject areas

  • 工程 (全部)

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