Molecular structure of two crystal forms of cyclic triadenylic acid at 1Å resolution

Yi Gui Gao, Howard Robinson, Yue Guan, Yen Chywan Liaw, Jacques H. van Boom, Gijs A. van der Marel, Andrew H.J. Wang

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5 引文 斯高帕斯(Scopus)


The three dimensional structures of cyclic deoxytriadenylic acid, c-d(ApApAp), from two different trigonal crystal forms (space groups P3 and R32) have been determined by x-ray diffraction analysis at 1Å resolution. Both structures were solved by direct methods and refined by anisotropic least squares refinement to R-factors of 0.109 and 0.137 for the P3 and R32 forms, respectively. In both crystal forms, each of the two independent c-d(ApApAp) molecules sits on the crystallographic 3-fold axis. All four independent c-d(ApApAp) molecules have similar backbone conformations. The deoxyriboses are in the S-type pucker with pseudorotation angles ranging from 156.7° to 168.6° and the bases have anti glycosyl torsion angles (χ falling in two ranges, one at −104.3° and the other ranging from −141.0° to −143.8°). In the R32 form, a hexahydrated cobalt(II) ion is found to coordinate through bridging water molecules to NI, N3, and N7 atoms of three adjacent adenines and oxygen atoms of phosphates. Comparison with other structures of cyclic oligonucleotides indicates that the sugar adopts N-type pucker in cyclic dinucleotides and S-type pucker in cyclic trinucleotides, regardless whether the sugar is a ribose or a deoxyribose.

頁(從 - 到)69-76
期刊Journal of Biomolecular Structure and Dynamics
出版狀態已發佈 - 8月 1998

ASJC Scopus subject areas

  • 結構生物學
  • 分子生物學


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