Molecular structure of nicked DNA: A substrate for DNA repair enzymes

J. Aymami, M. Coll, G. A. Van Der Marel, J. H. Van Boom, A. H.J. Wang, A. Rich

研究成果: 雜誌貢獻文章同行評審

61 引文 斯高帕斯(Scopus)

摘要

The molecular structure of a nicked dodecamer DNA double helix, made of a ternary system containing d(CGCGAAAACGCG) + d(CGCGTT) + d(TTCGCG) oligonucleotides, has been determined by x-ray diffraction analysis at 3 Å resolution. The molecule adopts a B-DNA conformation, not unlike those found in intact dodecamer DNA molecules crystallized in a somewhat different crystal lattice, despite a gap due to the absence of a phosphate group in the molecule. The helix has a distinct narrow minor groove near the center of the molecule at the AAAA region. This suggests that the internal stabilizing forces due to base stacking and hydrogen-bonding interactions are sufficient to overcome the loss of connectivity associated with the disruption of the covalent backbone of DNA.

原文英語
頁(從 - 到)2526-2530
頁數5
期刊Proceedings of the National Academy of Sciences of the United States of America
87
發行號7
DOIs
出版狀態已發佈 - 1990
對外發佈

ASJC Scopus subject areas

  • 多學科

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