Molecular mechanisms of apoptosis induced by magnolol in colon and liver cancer cells

Shyr Yi Lin, Yu Tza Chang, Jean-Dean Liu, Chung Hsun Yu, Yuan Soon Ho, Yi-Hsuan Lee, Wen Sen Lee

研究成果: 雜誌貢獻文章同行評審

72 引文 斯高帕斯(Scopus)

摘要

Magnolol has been reported to have anticancer activity. In this study we found that treatment with 100 μm magnolol induced apoptosis in cultured human hepatoma (Hep G2) and colon cancer (COLO 205) cell lines but not in human untransformed gingival fibroblasts and human umbilical vein endothelial cells. Our investigation of apoptosis in Hep G2 cells showed a sequence of associated intracellular events that included (a) increased cytosolic free Ca2+; (b) increased translocation of cytochrome c (Cyto c) from mitochondria to cytosol; (c) activation of caspase 3, caspase 8, and caspase 9; and (d) downregulation of bcl-2 protein. Pretreatment of the cells with the phospholipase C inhibitor 1-[6-[[(17β)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1 H-pyrrole-2,5-dione (U73122) or the intracellular chelator of Ca2+ 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid acetoxymethyl ester (BAPTA/AM) inhibited the subsequent magnolol augmentation of [ca2+]i and also the activation of caspase-8 and caspase-9, so that the occurrence of apoptosis in those cells was greatly reduced. Pretreatment of the cells with ZB4 (which disrupts the Fas response mechanism) also decreased the subsequent magnolol-induced caspase-8 activation and reduced the occurrence of apoptosis. We interpreted these findings to indicate that the above-listed sequence of intracellular events led to the apoptosis seen in Hep G2 cells and that [Ca2+]i, Cyto c, and Fas function as intracellular signals to coordinate those events.
原文英語
頁(從 - 到)73-83
頁數11
期刊Molecular Carcinogenesis
32
發行號2
DOIs
出版狀態已發佈 - 2001

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

指紋 深入研究「Molecular mechanisms of apoptosis induced by magnolol in colon and liver cancer cells」主題。共同形成了獨特的指紋。

引用此