Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan

Yu Ting Lin, Yu Ching Lan, Yen Ju Chen, Yu Hui Huang, Cheng Ming Lee, Tze Tze Liu, Wing Wai Wong, Jyh Yuan Yang, Chin Tien Wang, Yi Ming Arthur Chen

研究成果: 雜誌貢獻 › 文章

33 引文 (Scopus)

摘要

Background. Previously, we reported that there was an outbreak of human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC among injection drug users (IDUs) in Taiwan in 2004. The objectives of the present study were to conduct a molecular epidemiological analysis and to characterize the full-length genome of the Taiwanese CRF07_BC. Methods. Three hundred and fifty-eight patients with HIV-1/AIDS from hospitals and 133 HIV-1-infected inmates from detention centers were recruited. DNA sequencing and phylogenetic analysis were conducted to determine subtypes and evolutionary relationships. Recombination breakpoints of 2 full-length CRF07_BC strains were elucidated using a bootscanning method. Results. Of 206 HIV-1-infected patients who received a diagnosis in 2004, 44.7% were infected with subtype B, 53.4% with CRF07_BC, and 1.5% with CRF01_AE. Ninety-eight percent (109/111) of IDUs were infected with CRF07_BC. Deletions of 7-11 amino acids in both p6gag and p6pol proteins were noted among the Taiwanese CRF07_BC strains. The CRF_07BC strains belonged to 2 phylogenetic clusters, and the first cluster contained only CRF07_BC strains from the southern part of Taiwan. Conclusions. The Taiwanese CRF07_BC strains had 97% full-length sequence homology with the prototype from mainland China. CRF07_BC was first introduced into the southern region in 2002 and then spread to other regions in Taiwan in 2004.
原文英語
頁（從 - 到）1283-1293
頁數11
期刊Journal of Infectious Diseases
卷195
發行號9
DOIs
出版狀態已發佈 - 五月 1 2007
對外發佈Yes

指紋

Molecular Epidemiology
Virus Diseases
Drug Users
Taiwan
HIV-1
Injections
Sequence Homology
DNA Sequence Analysis
Genetic Recombination
Disease Outbreaks
China
Acquired Immunodeficiency Syndrome
Genome
Amino Acids
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

引用此文

Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan. / Lin, Yu Ting; Lan, Yu Ching; Chen, Yen Ju; Huang, Yu Hui; Lee, Cheng Ming; Liu, Tze Tze; Wong, Wing Wai; Yang, Jyh Yuan; Wang, Chin Tien; Chen, Yi Ming Arthur.

於: Journal of Infectious Diseases, 卷 195, 編號 9, 01.05.2007, p. 1283-1293.

研究成果: 雜誌貢獻 › 文章

Lin, YT, Lan, YC, Chen, YJ, Huang, YH, Lee, CM, Liu, TT, Wong, WW, Yang, JY, Wang, CT & Chen, YMA 2007, 'Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan', Journal of Infectious Diseases, 卷 195, 編號 9, 頁 1283-1293. https://doi.org/10.1086/513437
Lin YT, Lan YC, Chen YJ, Huang YH, Lee CM, Liu TT 等. Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan. Journal of Infectious Diseases. 2007 5月 1;195(9):1283-1293. https://doi.org/10.1086/513437
Lin, Yu Ting ; Lan, Yu Ching ; Chen, Yen Ju ; Huang, Yu Hui ; Lee, Cheng Ming ; Liu, Tze Tze ; Wong, Wing Wai ; Yang, Jyh Yuan ; Wang, Chin Tien ; Chen, Yi Ming Arthur. / Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan. 於: Journal of Infectious Diseases. 2007 ; 卷 195, 編號 9. 頁 1283-1293.
@article{3bfdf2ce4aca4310936120f3130fb539,
title = "Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan",
abstract = "Background. Previously, we reported that there was an outbreak of human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC among injection drug users (IDUs) in Taiwan in 2004. The objectives of the present study were to conduct a molecular epidemiological analysis and to characterize the full-length genome of the Taiwanese CRF07_BC. Methods. Three hundred and fifty-eight patients with HIV-1/AIDS from hospitals and 133 HIV-1-infected inmates from detention centers were recruited. DNA sequencing and phylogenetic analysis were conducted to determine subtypes and evolutionary relationships. Recombination breakpoints of 2 full-length CRF07_BC strains were elucidated using a bootscanning method. Results. Of 206 HIV-1-infected patients who received a diagnosis in 2004, 44.7{\%} were infected with subtype B, 53.4{\%} with CRF07_BC, and 1.5{\%} with CRF01_AE. Ninety-eight percent (109/111) of IDUs were infected with CRF07_BC. Deletions of 7-11 amino acids in both p6gag and p6pol proteins were noted among the Taiwanese CRF07_BC strains. The CRF_07BC strains belonged to 2 phylogenetic clusters, and the first cluster contained only CRF07_BC strains from the southern part of Taiwan. Conclusions. The Taiwanese CRF07_BC strains had 97{\%} full-length sequence homology with the prototype from mainland China. CRF07_BC was first introduced into the southern region in 2002 and then spread to other regions in Taiwan in 2004.",
author = "Lin, {Yu Ting} and Lan, {Yu Ching} and Chen, {Yen Ju} and Huang, {Yu Hui} and Lee, {Cheng Ming} and Liu, {Tze Tze} and Wong, {Wing Wai} and Yang, {Jyh Yuan} and Wang, {Chin Tien} and Chen, {Yi Ming Arthur}",
year = "2007",
month = "5",
day = "1",
doi = "10.1086/513437",
language = "English",
volume = "195",
pages = "1283--1293",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Molecular epidemiology of HIV-1 infection and full-length genomic analysis of circulating recombinant form 07_BC strains from injection drug users in Taiwan

AU - Lin, Yu Ting

AU - Lan, Yu Ching

AU - Chen, Yen Ju

AU - Huang, Yu Hui

AU - Lee, Cheng Ming

AU - Liu, Tze Tze

AU - Wong, Wing Wai

AU - Yang, Jyh Yuan

AU - Wang, Chin Tien

AU - Chen, Yi Ming Arthur

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Background. Previously, we reported that there was an outbreak of human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC among injection drug users (IDUs) in Taiwan in 2004. The objectives of the present study were to conduct a molecular epidemiological analysis and to characterize the full-length genome of the Taiwanese CRF07_BC. Methods. Three hundred and fifty-eight patients with HIV-1/AIDS from hospitals and 133 HIV-1-infected inmates from detention centers were recruited. DNA sequencing and phylogenetic analysis were conducted to determine subtypes and evolutionary relationships. Recombination breakpoints of 2 full-length CRF07_BC strains were elucidated using a bootscanning method. Results. Of 206 HIV-1-infected patients who received a diagnosis in 2004, 44.7% were infected with subtype B, 53.4% with CRF07_BC, and 1.5% with CRF01_AE. Ninety-eight percent (109/111) of IDUs were infected with CRF07_BC. Deletions of 7-11 amino acids in both p6gag and p6pol proteins were noted among the Taiwanese CRF07_BC strains. The CRF_07BC strains belonged to 2 phylogenetic clusters, and the first cluster contained only CRF07_BC strains from the southern part of Taiwan. Conclusions. The Taiwanese CRF07_BC strains had 97% full-length sequence homology with the prototype from mainland China. CRF07_BC was first introduced into the southern region in 2002 and then spread to other regions in Taiwan in 2004.

AB - Background. Previously, we reported that there was an outbreak of human immunodeficiency virus type 1 (HIV-1) circulating recombinant form (CRF) 07_BC among injection drug users (IDUs) in Taiwan in 2004. The objectives of the present study were to conduct a molecular epidemiological analysis and to characterize the full-length genome of the Taiwanese CRF07_BC. Methods. Three hundred and fifty-eight patients with HIV-1/AIDS from hospitals and 133 HIV-1-infected inmates from detention centers were recruited. DNA sequencing and phylogenetic analysis were conducted to determine subtypes and evolutionary relationships. Recombination breakpoints of 2 full-length CRF07_BC strains were elucidated using a bootscanning method. Results. Of 206 HIV-1-infected patients who received a diagnosis in 2004, 44.7% were infected with subtype B, 53.4% with CRF07_BC, and 1.5% with CRF01_AE. Ninety-eight percent (109/111) of IDUs were infected with CRF07_BC. Deletions of 7-11 amino acids in both p6gag and p6pol proteins were noted among the Taiwanese CRF07_BC strains. The CRF_07BC strains belonged to 2 phylogenetic clusters, and the first cluster contained only CRF07_BC strains from the southern part of Taiwan. Conclusions. The Taiwanese CRF07_BC strains had 97% full-length sequence homology with the prototype from mainland China. CRF07_BC was first introduced into the southern region in 2002 and then spread to other regions in Taiwan in 2004.

UR - http://www.scopus.com/inward/record.url?scp=34247537905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247537905&partnerID=8YFLogxK

U2 - 10.1086/513437

DO - 10.1086/513437

M3 - Article

C2 - 17396997

AN - SCOPUS:34247537905

VL - 195

SP - 1283

EP - 1293

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 9

ER -

存取文件