Molecular epidemiology and characterization of carbapenem-resistant klebsiella pneumoniae isolated from urine at a teaching hospital in Taiwan

Yuarn Jang Lee, Chih Hung Huang, Noor Andryan Ilsan, I. Hui Lee, Tzu Wen Huang

研究成果: 雜誌貢獻文章同行評審

摘要

Urinary tract infections (UTIs) are common in clinics and hospitals and are associated with a high economic burden. Enterobacterium Klebsiella pneumoniae is a prevalent agent causing UTIs. A high prevalence of carbapenem-resistant K. pneumoniae (CRKP) has emerged recently and is continuing to increase. Seventeen urinary CRKP isolates collected at a teaching hospital in Taiwan from December 2016 to September 2017 were analyzed to elucidate their drug resistance mechanisms. Two-thirds of the isolates were obtained from outpatients. Antimicrobial susceptibility tests demonstrated multidrug resistance in all the isolates. Multilocus sequence typing analysis showed high diversity among the isolates. PCR analysis demonstrated the presence of carbapenemases in three isolates. All isolates carried at least one other extended-spectrum β-lactamase, including TEM, DHA, and CTX-M. Fifteen isolates contained mutations in one of the outer membrane porins that were assessed. The expression levels of the acrB and/or oqxB efflux pump genes, as determined by qRT-PCR, were upregulated in 11 isolates. Six isolates might have utilized other efflux pumps or antimicrobial resistance mechanisms. These analyses demonstrated a highly diverse population and the presence of complex resistance mechanisms in urinary isolates of K. pneumoniae.
原文英語
文章編號271
頁(從 - 到)1-15
頁數15
期刊Microorganisms
9
發行號2
DOIs
出版狀態已發佈 - 二月 2021

ASJC Scopus subject areas

  • 微生物學
  • 病毒學
  • 微生物學(醫學)

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