Molecular cloning, primary structure, and characterization of two members of the mammalian electroneutral sodium-(potassium)-chloride cotransporter family expressed in kidney

Gerardo Gamba, Akihiko Miyanoshita, Michael Lombardi, Jonathan Lytton, Wen Sen Lee, Matthias A. Hediger, Steven C. Hebert

研究成果: 雜誌貢獻文章

491 引文 斯高帕斯(Scopus)

摘要

Electrically silent Na+-(K+)-Cl- transporter systems are present in a wide variety of cells and serve diverse physiological functions. In chloride secretory and absorbing epithelia, these cotransporters provide the chloride entry mechanism crucial for transcellular chloride transport. We have isolated cDNAs encoding the two major electroneutral sodium-chloride transporters present in the mammalian kidney, the bumetanide-sensitive Na+- K+-Cl- symporter and thiazide-sensitive Na+-Cl- cotransporter, and have characterized their functional activity in Xenopus laevis oocytes. Despite their differing sensitivities to bumetanide and thiazides and their different requirements for potassium, these ~115-kDa proteins share significant sequence similarity (~60%) and exhibit a topology featuring 12 potential membrane-spanning helices flanked by long non-hydrophobic domains at the NH2 and COOH termini. Northern blot analysis and in situ hybridization indicate that these transporters are expressed predominantly in kidney with an intrarenal distribution consistent with their recognized functional localization. These proteins establish a new family of Na+-(K+)-Cl- cotransporters.

原文英語
頁(從 - 到)17713-17722
頁數10
期刊Journal of Biological Chemistry
269
發行號26
出版狀態已發佈 - 七月 1 1994
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry

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