Molecular characterization of mungbean (Vigna radiata L.) starch branching enzyme I

Jia Wei Chang, Sing Chung Li, Yun Chi Shih, Reuben Wang, Pei Shan Chung, Yuan Tih Ko

研究成果: 雜誌貢獻文章

4 引文 斯高帕斯(Scopus)

摘要

Mungbean (Vigna radiata L. cv. Tainan no. 5) starch branching enzyme I (SBE, EC 2.4.1.18) cDNA, VrsbeI, was cloned, and its expression was characterized. Conserved regions of the family B SBE were used to amplify a full length cDNA of 2208 bp. Phylogeny was analyzed, and the partial 3D structure and functional features were predicted. Catalytic residues were identified in the (R/β)8-fold, and a unique loop from F365 to F376 between β3/α3 was located. Gene expression of VrsbeI in seeds during growth showed that the transcript appeared from week 1 and increased substantially at week 3-4. It was cloned into the pET30 vector and expressed in E. coli BL21(DE3) pLysS cells as a soluble recombinant protein. The affinity-purified recombinant VrSBEI exhibited a specific activity of 314.6 U/mg as an active enzyme with 114-fold activity enrichment from the crude extract.

原文英語
頁(從 - 到)10437-10444
頁數8
期刊Journal of Agricultural and Food Chemistry
58
發行號19
DOIs
出版狀態已發佈 - 十月 13 2010

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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