Molecular characterization of mungbean (Vigna radiata L.) starch branching enzyme I

Jia Wei Chang, Sing Chung Li, Yun Chi Shih, Reuben Wang, Pei Shan Chung, Yuan Tih Ko

研究成果: 雜誌貢獻文章

4 引文 (Scopus)

摘要

Mungbean (Vigna radiata L. cv. Tainan no. 5) starch branching enzyme I (SBE, EC 2.4.1.18) cDNA, VrsbeI, was cloned, and its expression was characterized. Conserved regions of the family B SBE were used to amplify a full length cDNA of 2208 bp. Phylogeny was analyzed, and the partial 3D structure and functional features were predicted. Catalytic residues were identified in the (R/β)8-fold, and a unique loop from F365 to F376 between β3/α3 was located. Gene expression of VrsbeI in seeds during growth showed that the transcript appeared from week 1 and increased substantially at week 3-4. It was cloned into the pET30 vector and expressed in E. coli BL21(DE3) pLysS cells as a soluble recombinant protein. The affinity-purified recombinant VrSBEI exhibited a specific activity of 314.6 U/mg as an active enzyme with 114-fold activity enrichment from the crude extract.

原文英語
頁(從 - 到)10437-10444
頁數8
期刊Journal of Agricultural and Food Chemistry
58
發行號19
DOIs
出版狀態已發佈 - 十月 13 2010

指紋

1,4-alpha-Glucan Branching Enzyme
1,4-alpha-glucan branching enzyme
Vigna radiata
mung beans
Complementary DNA
Phylogeny
Complex Mixtures
Recombinant Proteins
recombinant proteins
Gene expression
Escherichia coli
Seed
Seeds
Gene Expression
gene expression
phylogeny
extracts
Enzymes
Growth
enzymes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

引用此文

Molecular characterization of mungbean (Vigna radiata L.) starch branching enzyme I. / Chang, Jia Wei; Li, Sing Chung; Shih, Yun Chi; Wang, Reuben; Chung, Pei Shan; Ko, Yuan Tih.

於: Journal of Agricultural and Food Chemistry, 卷 58, 編號 19, 13.10.2010, p. 10437-10444.

研究成果: 雜誌貢獻文章

Chang, Jia Wei ; Li, Sing Chung ; Shih, Yun Chi ; Wang, Reuben ; Chung, Pei Shan ; Ko, Yuan Tih. / Molecular characterization of mungbean (Vigna radiata L.) starch branching enzyme I. 於: Journal of Agricultural and Food Chemistry. 2010 ; 卷 58, 編號 19. 頁 10437-10444.
@article{167445db0cd6410ca5ab7c0e57d620be,
title = "Molecular characterization of mungbean (Vigna radiata L.) starch branching enzyme I",
abstract = "Mungbean (Vigna radiata L. cv. Tainan no. 5) starch branching enzyme I (SBE, EC 2.4.1.18) cDNA, VrsbeI, was cloned, and its expression was characterized. Conserved regions of the family B SBE were used to amplify a full length cDNA of 2208 bp. Phylogeny was analyzed, and the partial 3D structure and functional features were predicted. Catalytic residues were identified in the (R/β)8-fold, and a unique loop from F365 to F376 between β3/α3 was located. Gene expression of VrsbeI in seeds during growth showed that the transcript appeared from week 1 and increased substantially at week 3-4. It was cloned into the pET30 vector and expressed in E. coli BL21(DE3) pLysS cells as a soluble recombinant protein. The affinity-purified recombinant VrSBEI exhibited a specific activity of 314.6 U/mg as an active enzyme with 114-fold activity enrichment from the crude extract.",
keywords = "Gene expression, Mungbean, Recombinant enzyme, Starch branching enzyme",
author = "Chang, {Jia Wei} and Li, {Sing Chung} and Shih, {Yun Chi} and Reuben Wang and Chung, {Pei Shan} and Ko, {Yuan Tih}",
year = "2010",
month = "10",
day = "13",
doi = "10.1021/jf102129f",
language = "English",
volume = "58",
pages = "10437--10444",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "19",

}

TY - JOUR

T1 - Molecular characterization of mungbean (Vigna radiata L.) starch branching enzyme I

AU - Chang, Jia Wei

AU - Li, Sing Chung

AU - Shih, Yun Chi

AU - Wang, Reuben

AU - Chung, Pei Shan

AU - Ko, Yuan Tih

PY - 2010/10/13

Y1 - 2010/10/13

N2 - Mungbean (Vigna radiata L. cv. Tainan no. 5) starch branching enzyme I (SBE, EC 2.4.1.18) cDNA, VrsbeI, was cloned, and its expression was characterized. Conserved regions of the family B SBE were used to amplify a full length cDNA of 2208 bp. Phylogeny was analyzed, and the partial 3D structure and functional features were predicted. Catalytic residues were identified in the (R/β)8-fold, and a unique loop from F365 to F376 between β3/α3 was located. Gene expression of VrsbeI in seeds during growth showed that the transcript appeared from week 1 and increased substantially at week 3-4. It was cloned into the pET30 vector and expressed in E. coli BL21(DE3) pLysS cells as a soluble recombinant protein. The affinity-purified recombinant VrSBEI exhibited a specific activity of 314.6 U/mg as an active enzyme with 114-fold activity enrichment from the crude extract.

AB - Mungbean (Vigna radiata L. cv. Tainan no. 5) starch branching enzyme I (SBE, EC 2.4.1.18) cDNA, VrsbeI, was cloned, and its expression was characterized. Conserved regions of the family B SBE were used to amplify a full length cDNA of 2208 bp. Phylogeny was analyzed, and the partial 3D structure and functional features were predicted. Catalytic residues were identified in the (R/β)8-fold, and a unique loop from F365 to F376 between β3/α3 was located. Gene expression of VrsbeI in seeds during growth showed that the transcript appeared from week 1 and increased substantially at week 3-4. It was cloned into the pET30 vector and expressed in E. coli BL21(DE3) pLysS cells as a soluble recombinant protein. The affinity-purified recombinant VrSBEI exhibited a specific activity of 314.6 U/mg as an active enzyme with 114-fold activity enrichment from the crude extract.

KW - Gene expression

KW - Mungbean

KW - Recombinant enzyme

KW - Starch branching enzyme

UR - http://www.scopus.com/inward/record.url?scp=78049292909&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78049292909&partnerID=8YFLogxK

U2 - 10.1021/jf102129f

DO - 10.1021/jf102129f

M3 - Article

C2 - 20822148

AN - SCOPUS:78049292909

VL - 58

SP - 10437

EP - 10444

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 19

ER -