Molecular analysis of mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexa

P. M. Chen, T. J. Chiou, I. T. Yu, F. S. Fan, C. J. Chu, S. C. Kao, W. S. Wang, J. H. Liu, Wen-Ming Hsu, M. H. Yang, T. C. Chao, C. J. Tai, L. T. Hsiao, J. T. Lin, C. C. Yen

研究成果: 雜誌貢獻文章

11 引文 斯高帕斯(Scopus)

摘要

Lymphomas of mucosa-associated lymphoid tissue (MALT) are a distinct subgroup of extranodal B-cell non-Hodgkin's lymphomas. Most studies have failed to demonstrate the clonal rearrangement of BCL-1, BCL-2 or c-MYC genes for MALT lymphomas. Further, alteration of the p53 gene is rarely demonstrated in low-grade MALT lymphomas, but can be detected in high-grade disease. Lymphomas of the ocular adnexa represent approximately eight percent of all extranodal lymphomas, most of which are MALT lymphomas, but few studies had explored the alterations of BCL-1, BCL-2, c-MYC and p53 genes specifically for ocular MALT lymphomas. We investigated the changes to BCL-1, BCL-2, c-MYC and p53 genes in these lymphomas for Taiwanese patients. Clonal rearrangement for immunoglobulin heavy-chain (IgH), BCL-1, BCL-2, c-MYC and p53 genes was examined for 16 cases of ocular MALT lymphoma. Restriction-length polymorphism and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) of the DNA, corresponding to exons 5 through 9, followed by DNA sequencing, were utilized to analyze the possible mutations of the p53 gene for these tumors. Thirteen of the cases revealed rearranged IgH genes using Southern blotting or PCR. No rearrangement of BCL-1, BCL-2, c-MYC or p53 genes was discovered, with point mutation of the p53 gene in one case. As for other types of MALT lymphomas, BCL-1, BCL-2 and c-MYC genes are not implicated in the pathogenesis of the ocular sub-group. Although alteration of the p53 gene is rare for low-grade ocular MALT lymphoma, its role in disease progression merits further research.

原文英語
頁(從 - 到)207-214
頁數8
期刊Leukemia and Lymphoma
42
發行號1-2
出版狀態已發佈 - 2001
對外發佈Yes

    指紋

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

引用此

Chen, P. M., Chiou, T. J., Yu, I. T., Fan, F. S., Chu, C. J., Kao, S. C., Wang, W. S., Liu, J. H., Hsu, W-M., Yang, M. H., Chao, T. C., Tai, C. J., Hsiao, L. T., Lin, J. T., & Yen, C. C. (2001). Molecular analysis of mucosa-associated lymphoid tissue (MALT) lymphoma of ocular adnexa. Leukemia and Lymphoma, 42(1-2), 207-214.