摘要
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 507-519 |
| 頁數 | 13 |
| 期刊 | Journal of Biomaterials Science, Polymer Edition |
| 卷 | 24 |
| 發行號 | 5 |
| DOIs | |
| 出版狀態 | 已發佈 - 四月 2013 |
| 對外發佈 | Yes |
指紋
ASJC Scopus subject areas
- Biophysics
- Biomaterials
- Bioengineering
- Biomedical Engineering
- Medicine(all)
引用此文
Modulation of the proliferation and matrix synthesis of chondrocytes by dynamic compression on genipin-crosslinked chitosan/collagen scaffolds. / And, Peng YuanWang; Tsai, Wei Bor.
於: Journal of Biomaterials Science, Polymer Edition, 卷 24, 編號 5, 04.2013, p. 507-519.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - Modulation of the proliferation and matrix synthesis of chondrocytes by dynamic compression on genipin-crosslinked chitosan/collagen scaffolds
AU - And, Peng YuanWang
AU - Tsai, Wei Bor
PY - 2013/4
Y1 - 2013/4
N2 - Dynamic compression is an important physical stimulus for the physiology of chondrocyte and articular cartilage tissue engineering. In this study, modulation of chondrocyte behaviors in chitosan/collagen scaffolds with different mechanical properties under free-swelling or dynamic compression conditions was investigated. Rabbit chondrocytes were seeded in chitosan/collagen scaffolds crosslinked by genipin (GP) with different concentrations, and then cultured for 3 days prior to cyclic compression of 40% strain, 0.1 Hz, and 30min/day for 2 weeks. The results showed that the cell proliferation was increased with increasing genipin concentrations and dynamic compression. On the other hand, although total glycosaminoglycans GAGs deposition was enhanced by dynamic compression under certain conditions, e.g. the GP0.5 chitosan/collagen scaffolds for 1 week of compression culture, normalized GAGs deposition per cell was decreased by dynamic compression. Our results suggest that while several studies suggest that dynamic compression benefits articular cartilage tissue engineering, many factors including scaffold types and compression conditions determine the outcome of dynamic compression culture.
AB - Dynamic compression is an important physical stimulus for the physiology of chondrocyte and articular cartilage tissue engineering. In this study, modulation of chondrocyte behaviors in chitosan/collagen scaffolds with different mechanical properties under free-swelling or dynamic compression conditions was investigated. Rabbit chondrocytes were seeded in chitosan/collagen scaffolds crosslinked by genipin (GP) with different concentrations, and then cultured for 3 days prior to cyclic compression of 40% strain, 0.1 Hz, and 30min/day for 2 weeks. The results showed that the cell proliferation was increased with increasing genipin concentrations and dynamic compression. On the other hand, although total glycosaminoglycans GAGs deposition was enhanced by dynamic compression under certain conditions, e.g. the GP0.5 chitosan/collagen scaffolds for 1 week of compression culture, normalized GAGs deposition per cell was decreased by dynamic compression. Our results suggest that while several studies suggest that dynamic compression benefits articular cartilage tissue engineering, many factors including scaffold types and compression conditions determine the outcome of dynamic compression culture.
KW - Chondrocytes
KW - Dynamic compression
KW - Elastic scaffold; glycosaminoglycan
KW - Mechanical property
UR - http://www.scopus.com/inward/record.url?scp=84880876192&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84880876192&partnerID=8YFLogxK
U2 - 10.1080/09205063.2012.696310
DO - 10.1080/09205063.2012.696310
M3 - Article
C2 - 23565864
AN - SCOPUS:84880876192
VL - 24
SP - 507
EP - 519
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
SN - 0920-5063
IS - 5
ER -