Modulation of the proliferation and matrix synthesis of chondrocytes by dynamic compression on genipin-crosslinked chitosan/collagen scaffolds

Peng YuanWang And, Wei Bor Tsai

研究成果: 雜誌貢獻文章

12 引文 (Scopus)

摘要

Dynamic compression is an important physical stimulus for the physiology of chondrocyte and articular cartilage tissue engineering. In this study, modulation of chondrocyte behaviors in chitosan/collagen scaffolds with different mechanical properties under free-swelling or dynamic compression conditions was investigated. Rabbit chondrocytes were seeded in chitosan/collagen scaffolds crosslinked by genipin (GP) with different concentrations, and then cultured for 3 days prior to cyclic compression of 40% strain, 0.1 Hz, and 30min/day for 2 weeks. The results showed that the cell proliferation was increased with increasing genipin concentrations and dynamic compression. On the other hand, although total glycosaminoglycans GAGs deposition was enhanced by dynamic compression under certain conditions, e.g. the GP0.5 chitosan/collagen scaffolds for 1 week of compression culture, normalized GAGs deposition per cell was decreased by dynamic compression. Our results suggest that while several studies suggest that dynamic compression benefits articular cartilage tissue engineering, many factors including scaffold types and compression conditions determine the outcome of dynamic compression culture.
原文英語
頁(從 - 到)507-519
頁數13
期刊Journal of Biomaterials Science, Polymer Edition
24
發行號5
DOIs
出版狀態已發佈 - 四月 2013
對外發佈Yes

指紋

Chitosan
Scaffolds (biology)
Chondrocytes
Collagen
Scaffolds
Modulation
Articular Cartilage
Tissue Engineering
Glycosaminoglycans
Cartilage
Tissue engineering
Cell Proliferation
Rabbits
Physiology
Cell proliferation
genipin
Swelling
Mechanical properties

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering
  • Medicine(all)

引用此文

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abstract = "Dynamic compression is an important physical stimulus for the physiology of chondrocyte and articular cartilage tissue engineering. In this study, modulation of chondrocyte behaviors in chitosan/collagen scaffolds with different mechanical properties under free-swelling or dynamic compression conditions was investigated. Rabbit chondrocytes were seeded in chitosan/collagen scaffolds crosslinked by genipin (GP) with different concentrations, and then cultured for 3 days prior to cyclic compression of 40{\%} strain, 0.1 Hz, and 30min/day for 2 weeks. The results showed that the cell proliferation was increased with increasing genipin concentrations and dynamic compression. On the other hand, although total glycosaminoglycans GAGs deposition was enhanced by dynamic compression under certain conditions, e.g. the GP0.5 chitosan/collagen scaffolds for 1 week of compression culture, normalized GAGs deposition per cell was decreased by dynamic compression. Our results suggest that while several studies suggest that dynamic compression benefits articular cartilage tissue engineering, many factors including scaffold types and compression conditions determine the outcome of dynamic compression culture.",
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