Modulation of osteogenic, adipogenic and myogenic differentiation of mesenchymal stem cells by submicron grooved topography

Peng Yuan Wang, Wen Tyng Li, Jiashing Yu, Wei Bor Tsai

研究成果: 雜誌貢獻文章同行評審

81 引文 斯高帕斯(Scopus)

摘要

Topographic cues have been recognized crucial on the modulation of cell behavior, and subsequent important for the design of implants, cell-based biomedical devices and tissue-engineered products. Grooved topography direct cells to align anisotropically on the substrates, resulting in an obvious morphological difference compared with the flat and the other topographies. This study aimed at investigating the effects of grooved topography on the differentiation of mesenchymal stem cells (MSCs) into osteoblasts, adipocytes and myoblasts. A series of submicron- grooved polystyrene substrates with equal grooveto- ridge ratio but different width and depth (width/depth (nm): 450/100, 450/350, 900/100, and 900/550) were fabricated based on electron beam lithography and soft lithography techniques. Primary rat MSCs (rMSCs) were cultured on these substrates without induction for differentiation for 6 days, and then subjected to induction for osteogenesis, adipogenesis and myogenesis. While the alignment of rMSCs strongly complied with the direction of the grooves and increased with groove depths, cell attachment on day 1 (̃1.5 × 104/cm2) and cell proliferation after 6 days of culture (̃5 × 104/cm2) were not significantly affected by substrate types. Osteogenesis, indicated by alkaline phosphatase activities and calcium deposit, was not significantly modulated by the grooved substrates, compared with the flat control, suggesting that cell alignment may not determine osteoinduction of rMSCs. On the other hand, adipogenesis, indicated by lipid production, was significantly enhanced by the grooved substrates compared with the flat surface (P<0.001). On the other hand, myogenesis, indicated by desmin and MHC staining, was enhanced by the grooves in a time- and groove size-dependent manner compared with the flat control. The results suggested that grooved topography has an in-depth potential for modulating the commitment of the stem cell lineages, which could benefit the development of advanced biomaterials for biomedical applications.
原文英語
頁(從 - 到)3015-3028
頁數14
期刊Journal of Materials Science: Materials in Medicine
23
發行號12
DOIs
出版狀態已發佈 - 十二月 2012
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物材料
  • 生物工程
  • 生物醫學工程

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