Modulation of human mesenchymal and pluripotent stem cell behavior using biophysical and biochemical cues: A review

Sheryl Ding, Peter Kingshott, Helmut Thissen, Martin Pera, Peng Yuan Wang

研究成果: 雜誌貢獻回顧型文獻

18 引文 (Scopus)

摘要

In vitro manipulation of human stem cells is a critical process in regenerative medicine and cellular therapies. Strategies and methods to maintain stem cells and direct them into specific lineages are ongoing challenges in these fields. To date, a number of studies have reported that besides biochemical stimulation, biophysical cues in the form of surface patterning and external stimulation also influence stem cell attachment, proliferation, and differentiation, and can be used in cell reprogramming and the maintenance of pluripotency. While biochemical cues are generally effective and easy to deliver, biophysical cues have many other advantages for scalability as they are cost efficient, have a longer lifetime, and can be easily defined. However, different protocols and cell sources utilized in a variety of studies have led to difficulties in obtaining clear conclusions about the effects of the biophysical environment on stem cells. In addition, the examination of different types of external stimulation is time consuming and limited by available fabrication techniques, resulting in a delay in commercialization and clinical applications. In this review, we aim to summarize the most important biophysical cues and methods for the culture of human stem cells, including mesenchymal and pluripotent stem cells, to facilitate their adoption in stem cell biology. The standard classical protocols of using biochemical cues will also be discussed for comparison. We believe that combining biochemical and biophysical stimulation has the greatest potential to generate functionally mature cells at a scalable and inexpensive rate for diverse applications in regenerative medicine and cell therapy. Biotechnol. Bioeng. 2017;114: 260–280.
原文英語
頁(從 - 到)260-280
頁數21
期刊Biotechnology and Bioengineering
114
發行號2
DOIs
出版狀態已發佈 - 二月 1 2017

指紋

Pluripotent Stem Cells
Stem cells
Mesenchymal Stromal Cells
Cues
Stem Cells
Modulation
Regenerative Medicine
Cytology
Cell- and Tissue-Based Therapy
Cell Biology
Cell Differentiation
Cell culture
Scalability
Maintenance
Cell Proliferation
Costs and Cost Analysis
Fabrication

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

引用此文

Modulation of human mesenchymal and pluripotent stem cell behavior using biophysical and biochemical cues : A review. / Ding, Sheryl; Kingshott, Peter; Thissen, Helmut; Pera, Martin; Wang, Peng Yuan.

於: Biotechnology and Bioengineering, 卷 114, 編號 2, 01.02.2017, p. 260-280.

研究成果: 雜誌貢獻回顧型文獻

Ding, Sheryl ; Kingshott, Peter ; Thissen, Helmut ; Pera, Martin ; Wang, Peng Yuan. / Modulation of human mesenchymal and pluripotent stem cell behavior using biophysical and biochemical cues : A review. 於: Biotechnology and Bioengineering. 2017 ; 卷 114, 編號 2. 頁 260-280.
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