Modulation of alignment, elongation and contraction of cardiomyocytes through a combination of nanotopography and rigidity of substrates

Peng Yuan Wang, Jiashing Yu, Jia Hua Lin, Wei Bor Tsai

研究成果: 雜誌貢獻文章

116 引文 斯高帕斯(Scopus)

摘要

The topographic and mechanical characteristics of engineered tissue constructs, simulating native tissues, should benefit tissue engineering. Previous studies reported that surface topography and substrate rigidity provide biomechanical cues to modulate cellular responses such as alignment, migration and differentiation. To fully address this issue, the present study aimed to examine the influence of nanogrooved substrates with different stiffnesses on the responses of rat cardiomyocytes. Nanogrooved substrates (450 nm in groove/ridge width; 100 or 350 nm in depth) made of polystyrene and polyurethane were prepared by imprinting from polydimethylsiloxane molds. The morphology and orientation of cardiomyocytes attached to the substrates were found to be influenced mainly by the nanogrooved structures, while the contractile function of the cells was regulated by the coupled effect of surface topography and substrate stiffness. The distribution of intracellular structural proteins such as vinculin and F-actin showed that the surface topography and substrate stiffness regulated the organization of the actin cytoskeleton and focal adhesion complexes, and consequently the contractile behavior of the cardiomyocytes. The beating rates of the cultured cardiomyocytes were dependent on both the surface topography and the substrate stiffness. The study provides insights into the interaction between cardiomyocytes and biomaterials, and benefits cardiac tissue engineering.
原文英語
頁(從 - 到)3285-3293
頁數9
期刊Acta Biomaterialia
7
發行號9
DOIs
出版狀態已發佈 - 九月 2011
對外發佈Yes

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

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