Modification of superoxide dismutase (SOD) mRNA and activity by a transient hypoxic stress in cultured glial cells

Chiang Shan Niu, Cheng Kuei Chang, Lieh Sheng Lin, Shuo B. Jou, Daih Huang Kuo, Shue S. Liao, Juei Tang Cheng

研究成果: 雜誌貢獻文章

10 引文 (Scopus)

摘要

In order to understand the role of superoxide dismutase (SOD) in response to transient hypoxia or hypoxia-reperfusion in astrocytes, the present study performed an in vitro investigation using rat glial cells in culture. Hypoxia was induced by an incubation with nitrogen gas for 10 min and that followed a further reperfusion with air for 10 min was indicating as hypoxia-normoxia. Activity of SOD was determined by the reduction of nitroblue tetrazolium (NTB). Changes of mRNA for Cu,Zn-SOD or Mn-SOD were also characterized using Northern blotting analysis. Transient hypoxia increased the activity of Mn-SOD but not that of Cu,Zn-SOD in glial cells. Expression of mRNA for SOD was also elevated in cells received hypoxia and the mRNA level for Mn-SOD raised higher than that for Cu,Zn-SOD. In cells received hypoxia-reperfusion, these changes of SOD both the activity and the mRNA level were not observed. Otherwise the SOD protein amount, both Cu,Zn- SOD and Mn-SOD, identified by Western blotting was not changed in glial cells receiving hypoxic stress or not. The obtained results suggest that gene expression and activity of Mn-SOD in glial cells can be activated in response to the transient hypoxic stress.
原文英語
頁(從 - 到)145-148
頁數4
期刊Neuroscience Letters
251
發行號3
DOIs
出版狀態已發佈 - 七月 31 1998
對外發佈Yes

指紋

Neuroglia
Superoxide Dismutase
Cultured Cells
Messenger RNA
Reperfusion
Cell Hypoxia
Nitroblue Tetrazolium
Astrocytes
Northern Blotting
Nitrogen
Cell Culture Techniques
Gases
Western Blotting
Air
Hypoxia
Gene Expression
Superoxide Dismutase-1

ASJC Scopus subject areas

  • Neuroscience(all)

引用此文

Modification of superoxide dismutase (SOD) mRNA and activity by a transient hypoxic stress in cultured glial cells. / Niu, Chiang Shan; Chang, Cheng Kuei; Lin, Lieh Sheng; Jou, Shuo B.; Kuo, Daih Huang; Liao, Shue S.; Cheng, Juei Tang.

於: Neuroscience Letters, 卷 251, 編號 3, 31.07.1998, p. 145-148.

研究成果: 雜誌貢獻文章

Niu, Chiang Shan ; Chang, Cheng Kuei ; Lin, Lieh Sheng ; Jou, Shuo B. ; Kuo, Daih Huang ; Liao, Shue S. ; Cheng, Juei Tang. / Modification of superoxide dismutase (SOD) mRNA and activity by a transient hypoxic stress in cultured glial cells. 於: Neuroscience Letters. 1998 ; 卷 251, 編號 3. 頁 145-148.
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abstract = "In order to understand the role of superoxide dismutase (SOD) in response to transient hypoxia or hypoxia-reperfusion in astrocytes, the present study performed an in vitro investigation using rat glial cells in culture. Hypoxia was induced by an incubation with nitrogen gas for 10 min and that followed a further reperfusion with air for 10 min was indicating as hypoxia-normoxia. Activity of SOD was determined by the reduction of nitroblue tetrazolium (NTB). Changes of mRNA for Cu,Zn-SOD or Mn-SOD were also characterized using Northern blotting analysis. Transient hypoxia increased the activity of Mn-SOD but not that of Cu,Zn-SOD in glial cells. Expression of mRNA for SOD was also elevated in cells received hypoxia and the mRNA level for Mn-SOD raised higher than that for Cu,Zn-SOD. In cells received hypoxia-reperfusion, these changes of SOD both the activity and the mRNA level were not observed. Otherwise the SOD protein amount, both Cu,Zn- SOD and Mn-SOD, identified by Western blotting was not changed in glial cells receiving hypoxic stress or not. The obtained results suggest that gene expression and activity of Mn-SOD in glial cells can be activated in response to the transient hypoxic stress.",
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