Moderate intake of red wine improves ischemia-induced neovascularization in diabetic mice-Roles of endothelial progenitor cells and nitric oxide

Po Hsun Huang, Hsiao Ya Tsai, Chao Hung Wang, Yung Hsiang Chen, Jia Shiong Chen, Feng Yen Lin, Chih Pei Lin, Tao Cheng Wu, Masataka Sata, Jaw Wen Chen, Shing Jong Lin

研究成果: 雜誌貢獻文章

34 引文 (Scopus)

摘要

Objective: Circulating endothelial progenitor cells (EPCs) play a significant role in postnatal neovascularization. Patients with diabetes have attenuated EPC functions and impaired angiogenic response after tissue ischemia. We investigated whether moderate red wine consumption can enhance blood flow recovery in response to tissue ischemia by enhancement of EPC functions in diabetic mice. Methods and results: Starting at 4 weeks after diabetes onset, red wine (4ml/kg/day) or ethanol were administered to streptozotocin (STZ)-induced (type 1) diabetic mice and KKAy-Ta (type 2) mice. Unilateral hind limb ischemia surgery was conducted after 2 weeks of red wine or ethanol ingestion. Type 1 and type 2 diabetic mice given red wine, but not ethanol, had significantly increased collateral flow about 30% and augmented capillary density in ischemic tissues. These beneficial effects were markedly abolished by an eNOS inhibitor (L-NAME). Flow cytometry analysis showed impaired EPC-like cells (Sca-1+/Flk-1+) mobilization after ischemia surgery in diabetic mice, but augmented mobilization in red wine group (baseline vs. 2 days after operation: 0.88±0.06% vs. 1.73±0.29%, p=0.010). C-kit positive bone marrow cells isolated from diabetic mice given red wine had enhanced adhesion and migration compared to mice given vehicle. By in-vitro studies, incubation with red wine in high-glucose medium significantly reduced H2O2 production, and improved high glucose-suppressed EPC functions by nitric oxide-related mechanisms. Conclusions: Our findings demonstrate that red wine consumption enhances blood flow recovery after tissue ischemia in diabetic mice. These effects may partly derive from enhanced EPC functions by upregulation of eNOS activity.

原文英語
頁(從 - 到)426-435
頁數10
期刊Atherosclerosis
212
發行號2
DOIs
出版狀態已發佈 - 十月 2010

指紋

Wine
Nitric Oxide
Ischemia
Ethanol
Glucose
Endothelial Progenitor Cells
NG-Nitroarginine Methyl Ester
Streptozocin
Bone Marrow Cells
Flow Cytometry
Up-Regulation
Extremities
Eating

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

引用此文

Moderate intake of red wine improves ischemia-induced neovascularization in diabetic mice-Roles of endothelial progenitor cells and nitric oxide. / Huang, Po Hsun; Tsai, Hsiao Ya; Wang, Chao Hung; Chen, Yung Hsiang; Chen, Jia Shiong; Lin, Feng Yen; Lin, Chih Pei; Wu, Tao Cheng; Sata, Masataka; Chen, Jaw Wen; Lin, Shing Jong.

於: Atherosclerosis, 卷 212, 編號 2, 10.2010, p. 426-435.

研究成果: 雜誌貢獻文章

Huang, Po Hsun ; Tsai, Hsiao Ya ; Wang, Chao Hung ; Chen, Yung Hsiang ; Chen, Jia Shiong ; Lin, Feng Yen ; Lin, Chih Pei ; Wu, Tao Cheng ; Sata, Masataka ; Chen, Jaw Wen ; Lin, Shing Jong. / Moderate intake of red wine improves ischemia-induced neovascularization in diabetic mice-Roles of endothelial progenitor cells and nitric oxide. 於: Atherosclerosis. 2010 ; 卷 212, 編號 2. 頁 426-435.
@article{4cd87621b1cf4211937ee8eb321be685,
title = "Moderate intake of red wine improves ischemia-induced neovascularization in diabetic mice-Roles of endothelial progenitor cells and nitric oxide",
abstract = "Objective: Circulating endothelial progenitor cells (EPCs) play a significant role in postnatal neovascularization. Patients with diabetes have attenuated EPC functions and impaired angiogenic response after tissue ischemia. We investigated whether moderate red wine consumption can enhance blood flow recovery in response to tissue ischemia by enhancement of EPC functions in diabetic mice. Methods and results: Starting at 4 weeks after diabetes onset, red wine (4ml/kg/day) or ethanol were administered to streptozotocin (STZ)-induced (type 1) diabetic mice and KKAy-Ta (type 2) mice. Unilateral hind limb ischemia surgery was conducted after 2 weeks of red wine or ethanol ingestion. Type 1 and type 2 diabetic mice given red wine, but not ethanol, had significantly increased collateral flow about 30{\%} and augmented capillary density in ischemic tissues. These beneficial effects were markedly abolished by an eNOS inhibitor (L-NAME). Flow cytometry analysis showed impaired EPC-like cells (Sca-1+/Flk-1+) mobilization after ischemia surgery in diabetic mice, but augmented mobilization in red wine group (baseline vs. 2 days after operation: 0.88±0.06{\%} vs. 1.73±0.29{\%}, p=0.010). C-kit positive bone marrow cells isolated from diabetic mice given red wine had enhanced adhesion and migration compared to mice given vehicle. By in-vitro studies, incubation with red wine in high-glucose medium significantly reduced H2O2 production, and improved high glucose-suppressed EPC functions by nitric oxide-related mechanisms. Conclusions: Our findings demonstrate that red wine consumption enhances blood flow recovery after tissue ischemia in diabetic mice. These effects may partly derive from enhanced EPC functions by upregulation of eNOS activity.",
keywords = "Diabetes, Endothelial progenitor cells, Nitric oxide, Red wine",
author = "Huang, {Po Hsun} and Tsai, {Hsiao Ya} and Wang, {Chao Hung} and Chen, {Yung Hsiang} and Chen, {Jia Shiong} and Lin, {Feng Yen} and Lin, {Chih Pei} and Wu, {Tao Cheng} and Masataka Sata and Chen, {Jaw Wen} and Lin, {Shing Jong}",
year = "2010",
month = "10",
doi = "10.1016/j.atherosclerosis.2010.06.034",
language = "English",
volume = "212",
pages = "426--435",
journal = "Atherosclerosis",
issn = "0021-9150",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Moderate intake of red wine improves ischemia-induced neovascularization in diabetic mice-Roles of endothelial progenitor cells and nitric oxide

AU - Huang, Po Hsun

AU - Tsai, Hsiao Ya

AU - Wang, Chao Hung

AU - Chen, Yung Hsiang

AU - Chen, Jia Shiong

AU - Lin, Feng Yen

AU - Lin, Chih Pei

AU - Wu, Tao Cheng

AU - Sata, Masataka

AU - Chen, Jaw Wen

AU - Lin, Shing Jong

PY - 2010/10

Y1 - 2010/10

N2 - Objective: Circulating endothelial progenitor cells (EPCs) play a significant role in postnatal neovascularization. Patients with diabetes have attenuated EPC functions and impaired angiogenic response after tissue ischemia. We investigated whether moderate red wine consumption can enhance blood flow recovery in response to tissue ischemia by enhancement of EPC functions in diabetic mice. Methods and results: Starting at 4 weeks after diabetes onset, red wine (4ml/kg/day) or ethanol were administered to streptozotocin (STZ)-induced (type 1) diabetic mice and KKAy-Ta (type 2) mice. Unilateral hind limb ischemia surgery was conducted after 2 weeks of red wine or ethanol ingestion. Type 1 and type 2 diabetic mice given red wine, but not ethanol, had significantly increased collateral flow about 30% and augmented capillary density in ischemic tissues. These beneficial effects were markedly abolished by an eNOS inhibitor (L-NAME). Flow cytometry analysis showed impaired EPC-like cells (Sca-1+/Flk-1+) mobilization after ischemia surgery in diabetic mice, but augmented mobilization in red wine group (baseline vs. 2 days after operation: 0.88±0.06% vs. 1.73±0.29%, p=0.010). C-kit positive bone marrow cells isolated from diabetic mice given red wine had enhanced adhesion and migration compared to mice given vehicle. By in-vitro studies, incubation with red wine in high-glucose medium significantly reduced H2O2 production, and improved high glucose-suppressed EPC functions by nitric oxide-related mechanisms. Conclusions: Our findings demonstrate that red wine consumption enhances blood flow recovery after tissue ischemia in diabetic mice. These effects may partly derive from enhanced EPC functions by upregulation of eNOS activity.

AB - Objective: Circulating endothelial progenitor cells (EPCs) play a significant role in postnatal neovascularization. Patients with diabetes have attenuated EPC functions and impaired angiogenic response after tissue ischemia. We investigated whether moderate red wine consumption can enhance blood flow recovery in response to tissue ischemia by enhancement of EPC functions in diabetic mice. Methods and results: Starting at 4 weeks after diabetes onset, red wine (4ml/kg/day) or ethanol were administered to streptozotocin (STZ)-induced (type 1) diabetic mice and KKAy-Ta (type 2) mice. Unilateral hind limb ischemia surgery was conducted after 2 weeks of red wine or ethanol ingestion. Type 1 and type 2 diabetic mice given red wine, but not ethanol, had significantly increased collateral flow about 30% and augmented capillary density in ischemic tissues. These beneficial effects were markedly abolished by an eNOS inhibitor (L-NAME). Flow cytometry analysis showed impaired EPC-like cells (Sca-1+/Flk-1+) mobilization after ischemia surgery in diabetic mice, but augmented mobilization in red wine group (baseline vs. 2 days after operation: 0.88±0.06% vs. 1.73±0.29%, p=0.010). C-kit positive bone marrow cells isolated from diabetic mice given red wine had enhanced adhesion and migration compared to mice given vehicle. By in-vitro studies, incubation with red wine in high-glucose medium significantly reduced H2O2 production, and improved high glucose-suppressed EPC functions by nitric oxide-related mechanisms. Conclusions: Our findings demonstrate that red wine consumption enhances blood flow recovery after tissue ischemia in diabetic mice. These effects may partly derive from enhanced EPC functions by upregulation of eNOS activity.

KW - Diabetes

KW - Endothelial progenitor cells

KW - Nitric oxide

KW - Red wine

UR - http://www.scopus.com/inward/record.url?scp=77957701546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957701546&partnerID=8YFLogxK

U2 - 10.1016/j.atherosclerosis.2010.06.034

DO - 10.1016/j.atherosclerosis.2010.06.034

M3 - Article

C2 - 20637466

AN - SCOPUS:77957701546

VL - 212

SP - 426

EP - 435

JO - Atherosclerosis

JF - Atherosclerosis

SN - 0021-9150

IS - 2

ER -