Model of the αLβ2 integrin I-domain/ICAM-1 DI interface suggests that subtle changes in loop orientation determine ligand specificity

Glen B. Legge, Garrett M. Morris, Michel F. Sanner, Yoshikazu Takada, Arthur J. Olson, Flavio Grynszpan

研究成果: 雜誌貢獻文章

14 引文 (Scopus)

摘要

The interaction of the αLβ2 integrin with its cellular ligand the intercellular adhesion molecule-1 (ICAM-1) is critical for the tight binding interaction between most leukocytes and the vascular endothelium before transendothelial migration to the sites of inflammation. In this article we have modeled the αL subunit I-domain in its active form, which was computationally docked with the D1 domain of the ICAM-1 to probe potential protein-protein interactions. The experimentally observed key interaction between the carboxylate of Glu 34 in the ICAM-1 D1 domain and the metal ion-dependent adhesion site (MIDAS) in the open αL I-domain was consistently reproduced by our calculations. The calculations reveal the nature of the αLβ2/ICAM-1 interaction and suggest an explanation for the increased ligand-binding affinity in the "open" versus the "closed" conformation of the αL I-domain. A mechanism for substrate selectivity among αL, αM, and α2 I-domains is suggested whereby the orientation of the loops within the I-domain is critical in mediating the interaction of the Glu 34 carboxylate of ICAM-1 D1 with the MIDAS.

原文英語
頁(從 - 到)151-160
頁數10
期刊Proteins: Structure, Function and Genetics
48
發行號2
DOIs
出版狀態已發佈 - 八月 1 2002
對外發佈Yes

指紋

Intercellular Adhesion Molecule-1
Integrins
Ligands
Metal ions
Adhesion
Metals
Ions
Transendothelial and Transepithelial Migration
Vascular Endothelium
Conformations
Proteins
Leukocytes
Inflammation
Substrates

ASJC Scopus subject areas

  • Genetics
  • Structural Biology
  • Biochemistry

引用此文

Model of the αLβ2 integrin I-domain/ICAM-1 DI interface suggests that subtle changes in loop orientation determine ligand specificity. / Legge, Glen B.; Morris, Garrett M.; Sanner, Michel F.; Takada, Yoshikazu; Olson, Arthur J.; Grynszpan, Flavio.

於: Proteins: Structure, Function and Genetics, 卷 48, 編號 2, 01.08.2002, p. 151-160.

研究成果: 雜誌貢獻文章

Legge, Glen B. ; Morris, Garrett M. ; Sanner, Michel F. ; Takada, Yoshikazu ; Olson, Arthur J. ; Grynszpan, Flavio. / Model of the αLβ2 integrin I-domain/ICAM-1 DI interface suggests that subtle changes in loop orientation determine ligand specificity. 於: Proteins: Structure, Function and Genetics. 2002 ; 卷 48, 編號 2. 頁 151-160.
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