MMP2-sensing up-conversion nanoparticle for fluorescence biosensing in head and neck cancer cells

Yung Chieh Chan, Chieh Wei Chen, Ming Hsien Chan, Yu Chan Chang, Wei Min Chang, Li Hsing Chi, Hui Ming Yu, Yuan Feng Lin, Din Ping Tsai, Ru Shi Liu, Michael Hsiao

研究成果: 雜誌貢獻文章同行評審

32 引文 斯高帕斯(Scopus)

摘要

Upconversion nanoparticles (UCNPs) have extensive biological-applications because of their bio-compatibility, tunable optical properties and their ability to be excited by infrared radiation. Matrix metalloproteinases (MMPs) play important roles in extracellular matrix remodelling; they are usually found to significantly increase during cancer progression, and these increases may lead to poor patient survival. In this study, we produced a biosensor that can be recognized by MMP2 and then be unravelled by the attached quencher to emit visible light. We used 3.5-nm gold nanoparticles as a quencher that absorbed emission from UCNPs at a wavelength of 540nm. The biosensor consists of an upconversion nanoparticle, MMP2-recognized polypeptides and quenchers. Here, UCNPs consisting of NaYF4:Yb3+/Er3+ were prepared via a high temperature co-precipitation method while protecting the oleic acid ligand. To improve the biocompatibility and modify the UCNPs with a polypeptide, they were coated with a silica shell and further conjugated with MMP-recognizing polypeptides. The polypeptide has two ends of featuring carboxylic and thiol groups that react with UCNPs and AuNPs, and the resulting nanoparticles were referred to as UCNP@p-Au. According to the in vitro cell viability analysis, UCNP@p-Au exhibited little toxicity and biocompatibility in head and neck cancer cells. Cellular uptake studies showed that the MMP-based biosensor was activated by 980-nm irradiation to emit green light. This MMP-based biosensor may serve as sensitive and specific molecular fluorescent probe in biological-applications.

原文英語
頁(從 - 到)131-139
頁數9
期刊Biosensors and Bioelectronics
80
DOIs
出版狀態已發佈 - 六月 15 2016

ASJC Scopus subject areas

  • 生物物理學
  • 生物醫學工程
  • 生物技術
  • 電化學

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