Mixed infection with Beijing and non-Beijing strains in pulmonary tuberculosis in Taiwan: Prevalence, risk factors, and dominant strain

J. Y. Wang, H. L. Hsu, M. C. Yu, C. Y. Chiang, F. L. Yu, C. J. Yu, L. N. Lee, P. C. Yang

研究成果: 雜誌貢獻文章

19 引文 (Scopus)

摘要

Patients with pulmonary tuberculosis (TB) can be simultaneously infected with different strains of Mycobacterium tuberculosis (mixed infection). We investigated the prevalence and risk factors of mixed infection by Beijing and non-Beijing strains in pulmonary TB patients in Taiwan. We developed a quantitative PCR method to simultaneously detect the presence of Beijing and non-Beijing strains. A total of 868 pretreatment samples (from 868 patients), including 563 sputum samples smear-positive for acid-fast bacilli and 305 liquid medium samples culture-positive for mycobacteria, were tested. Medical records of patients with culture-confirmed pulmonary TB were reviewed. The detection limit of our quantitative PCR method was five copies of target sequences. With mycobacterial culture result as the reference standard, the sensitivity and specificity of our quantitative PCR method were 95% and 98%, respectively. M. tuberculosis strains were isolated in 466 samples, of which 231 (49.6%) were infected with a Beijing strain. Another 14 patients (3.0%) had mixed infection, with the Beijing strain being the dominant strain in 13 (93%). Age
原文英語
頁(從 - 到)1239-1245
頁數7
期刊Clinical Microbiology and Infection
17
發行號8
DOIs
出版狀態已發佈 - 八月 2011

指紋

Coinfection
Taiwan
Pulmonary Tuberculosis
Mycobacterium tuberculosis
Polymerase Chain Reaction
Mycobacterium
Sputum
Bacillus
Medical Records
Culture Media
Limit of Detection
Beijing
Sensitivity and Specificity
Acids

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

引用此文

Mixed infection with Beijing and non-Beijing strains in pulmonary tuberculosis in Taiwan : Prevalence, risk factors, and dominant strain. / Wang, J. Y.; Hsu, H. L.; Yu, M. C.; Chiang, C. Y.; Yu, F. L.; Yu, C. J.; Lee, L. N.; Yang, P. C.

於: Clinical Microbiology and Infection, 卷 17, 編號 8, 08.2011, p. 1239-1245.

研究成果: 雜誌貢獻文章

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