The morphological and ultrastructural alterations of skeletal muscle in experimental rats with heat stress were investigated. Fifteen male Sprague-Dawley rats were exposed in a 42 degrees C constant temperature oven, resulting in a heat stress state; ten rats were used as controls. All treated rats had weakness of the 4 limbs associated with increased serum creatine kinase levels (p <0.01). Soleus muscles were submitted to histological, histochemical, ultrastructural and quantitative-morphometric analysis. The group receiving heat stress showed many ragged-red fibers in Gomori trichrome stain and appeared hyper-reactive in succinate dehydrogenase and cytochrome C oxidase stains. The ultrastructure of ragged-red fibers showed increased mitochondrial aggregation as multiple small nests, which were particularly located in the subsarcolemmal space. The mitochondrial area was significantly increased in heat stress rats (p <0.001). The consistently increased mitochondrial area and histochemical alterations of mitochondria are early pathological abnormalities in muscles with heat stress and indicate fundamental impairment of energy metabolism.
|頁（從 - 到）||233-239|
|期刊||Proceedings of the National Science Council, Republic of China. Part B, Life sciences|
|出版狀態||已發佈 - 十月 1995|