TY - JOUR
T1 - miR-509 inhibits cancer stemness properties in oral carcinomas via directly targeting PlK1
AU - Lu, Ming Yi
AU - Fang, Chih Yuan
AU - Hsieh, Pei Ling
AU - Liao, Yi Wen
AU - Tsai, Lo Lin
AU - Yu, Cheng Chia
N1 - Funding Information:
This study was supported by grants from Chung Shan Medical University Hospital ( CSH-2019-C-029 ) and Wan Fang Hospital ( 109-wf-phd-02 ) in Taiwan.
Publisher Copyright:
© 2021 Association for Dental Sciences of the Republic of China
PY - 2022/4
Y1 - 2022/4
N2 - Background/purpose: Oral cancer is one of the common cancers worldwide. Emerging evidence has indicated that microRNAs (non-coding RNA molecules of approximately 22 nucleotides in length) are implicated in the regulation of cancer stemness. However, the functional role of microRNA-509 (miR-509) in the characteristics of oral cancer stem cells (CSCs) has not been unraveled. Materials and methods: The expression level of miR-509 in ALDH1+ and sphere oral CSCs was examined by qRT-PCR. The aldehyde dehydrogenase 1 (ALDH1) activity and CD44 expression were assessed using flow cytometry. Self-renewal, transwell migration, and colony formation assays were conducted to measure the CSC phenotypes. Besides, a luciferase reporter assay was used to confirm the direct interaction between miR-509 and its target polo-like kinase 1 (plk1). Results: We showed the expression of miR-509 was downregulated in the CSCs derived from oral cancer cells (SAS), and upregulation of miR-509 diminished the several CSCs features, including ALDH1 activity, self-renewal capacity, CD44 expression, migration, and colony-forming abilities. Moreover, the result from the luciferase reporter assay validated the direct binding of miR-509 to plk1. Conclusion: Our results suggest that the miR-509/plk1 axis may mediate the cancer stemness in oral cancer, and targeting this axis may attenuate the progression of oral cancer.
AB - Background/purpose: Oral cancer is one of the common cancers worldwide. Emerging evidence has indicated that microRNAs (non-coding RNA molecules of approximately 22 nucleotides in length) are implicated in the regulation of cancer stemness. However, the functional role of microRNA-509 (miR-509) in the characteristics of oral cancer stem cells (CSCs) has not been unraveled. Materials and methods: The expression level of miR-509 in ALDH1+ and sphere oral CSCs was examined by qRT-PCR. The aldehyde dehydrogenase 1 (ALDH1) activity and CD44 expression were assessed using flow cytometry. Self-renewal, transwell migration, and colony formation assays were conducted to measure the CSC phenotypes. Besides, a luciferase reporter assay was used to confirm the direct interaction between miR-509 and its target polo-like kinase 1 (plk1). Results: We showed the expression of miR-509 was downregulated in the CSCs derived from oral cancer cells (SAS), and upregulation of miR-509 diminished the several CSCs features, including ALDH1 activity, self-renewal capacity, CD44 expression, migration, and colony-forming abilities. Moreover, the result from the luciferase reporter assay validated the direct binding of miR-509 to plk1. Conclusion: Our results suggest that the miR-509/plk1 axis may mediate the cancer stemness in oral cancer, and targeting this axis may attenuate the progression of oral cancer.
KW - Cancer stem cells
KW - MicroRNA-509
KW - Oral cancer
KW - Polo like kinase 1
UR - http://www.scopus.com/inward/record.url?scp=85116732266&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85116732266&partnerID=8YFLogxK
U2 - 10.1016/j.jds.2021.09.028
DO - 10.1016/j.jds.2021.09.028
M3 - Article
AN - SCOPUS:85116732266
VL - 17
SP - 653
EP - 658
JO - Journal of Dental Sciences
JF - Journal of Dental Sciences
SN - 1991-7902
IS - 2
ER -