Minipool Caprylic Acid Fractionation of Plasma Using Disposable Equipment: A Practical Method to Enhance Immunoglobulin Supply in Developing Countries

Magdy El-Ekiaby, Mariángela Vargas, Makram Sayed, George Gorgy, Hadi Goubran, Mirjana Radosevic, Thierry Burnouf

研究成果: 雜誌貢獻文章

10 引文 (Scopus)

摘要

Background Immunoglobulin G (IgG) is an essential plasma-derived medicine that is lacking in developing countries. IgG shortages leave immunodeficient patients without treatment, exposing them to devastating recurrent infections from local pathogens. A simple and practical method for producing IgG from normal or convalescent plasma collected in developing countries is needed to provide better, faster access to IgG for patients in need. Methodology/Principal Findings IgG was purified from 10 consecutive minipools of 20 plasma donations collected in Egypt using single-use equipment. Plasma donations in their collection bags were subjected to 5%-pH5.5 caprylic acid treatment for 90 min at 31°C, and centrifuged to remove the precipitate. Supernatants were pooled, then dialyzed and concentrated using a commercial disposable hemodialyzer. The final preparation was filtered online by gravity, aseptically dispensed into storage transfusion bags, and frozen at 5 logs reduction of HIV, BVDV, and PRV infectivity in less than 15 min of caprylic acid treatment. Conclusions/Significance 90% pure, virally-inactivated immunoglobulins can be prepared from plasma minipools using simple disposable equipment and bag systems. This easy-to-implement process could be used to produce immunoglobulins from local plasma in developing countries to treat immunodeficient patients. It is also relevant for preparing hyperimmune IgG from convalescent plasma during infectious outbreaks such as the current Ebola virus episode.
原文英語
文章編號e0003501
期刊PLoS Neglected Tropical Diseases
9
發行號2
DOIs
出版狀態已發佈 - 2015

指紋

Disposable Equipment
Developing Countries
Immunoglobulins
Immunoglobulin G
Ebolavirus
Artificial Kidneys
Egypt
Gravitation
octanoic acid
Disease Outbreaks
Therapeutics
Medicine
HIV
Equipment and Supplies

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

引用此文

Minipool Caprylic Acid Fractionation of Plasma Using Disposable Equipment : A Practical Method to Enhance Immunoglobulin Supply in Developing Countries. / El-Ekiaby, Magdy; Vargas, Mariángela; Sayed, Makram; Gorgy, George; Goubran, Hadi; Radosevic, Mirjana; Burnouf, Thierry.

於: PLoS Neglected Tropical Diseases, 卷 9, 編號 2, e0003501, 2015.

研究成果: 雜誌貢獻文章

El-Ekiaby, Magdy ; Vargas, Mariángela ; Sayed, Makram ; Gorgy, George ; Goubran, Hadi ; Radosevic, Mirjana ; Burnouf, Thierry. / Minipool Caprylic Acid Fractionation of Plasma Using Disposable Equipment : A Practical Method to Enhance Immunoglobulin Supply in Developing Countries. 於: PLoS Neglected Tropical Diseases. 2015 ; 卷 9, 編號 2.
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abstract = "Background Immunoglobulin G (IgG) is an essential plasma-derived medicine that is lacking in developing countries. IgG shortages leave immunodeficient patients without treatment, exposing them to devastating recurrent infections from local pathogens. A simple and practical method for producing IgG from normal or convalescent plasma collected in developing countries is needed to provide better, faster access to IgG for patients in need. Methodology/Principal Findings IgG was purified from 10 consecutive minipools of 20 plasma donations collected in Egypt using single-use equipment. Plasma donations in their collection bags were subjected to 5{\%}-pH5.5 caprylic acid treatment for 90 min at 31°C, and centrifuged to remove the precipitate. Supernatants were pooled, then dialyzed and concentrated using a commercial disposable hemodialyzer. The final preparation was filtered online by gravity, aseptically dispensed into storage transfusion bags, and frozen at 5 logs reduction of HIV, BVDV, and PRV infectivity in less than 15 min of caprylic acid treatment. Conclusions/Significance 90{\%} pure, virally-inactivated immunoglobulins can be prepared from plasma minipools using simple disposable equipment and bag systems. This easy-to-implement process could be used to produce immunoglobulins from local plasma in developing countries to treat immunodeficient patients. It is also relevant for preparing hyperimmune IgG from convalescent plasma during infectious outbreaks such as the current Ebola virus episode.",
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