MicroRNA regulation via DNA methylation during the morula to blastocyst transition in mice

Yee Ming Lee, Huei Wen Chen, Pawan Kumar Maurya, Ching Mao Su, Chii Ruey Tzeng

研究成果: 雜誌貢獻文章

13 引文 (Scopus)

摘要

Epigenetic regulation is responsible for transcriptional silencing of genes and parental imprinting. This study addresses the question whether microRNAs (miRNAs) could be affected by DNA methylation during morula-blastocyst transition. Mouse embryos were treated with/without a DNA methyltransferase inhibitor (5-aza-2′-deoxycytidine, 5-aza-dC, 10 nM-5 μM). Changes of miRNAs were analyzed by quantitative real-time (Q-PCR)-based megaplex pre-amp microRNA assays. Development from morula to blastocyst in mice was inhibited by 5-aza-dC in a dose-dependent manner (10 nM-5 μM), with half of the embryos arrested at morula stage when treated with levels of 5-aza-dC as low as 50 nM. In total, 48 down-regulated microRNAs and 17 up-regulated microRNAs (≥5-fold changes) were identified after 5-aza-dC treatment, including let-7e, mir-20a, mir-21, mir-34b, mir-128b and mir-452. Their predicted targets were selected based on software analysis, published databases and further confirmed by Q-PCR. At least eight targets, including dnmt3a, jagged 1, sp1, edg2, abcg4, numa1, tmsb10 and csf1r were confirmed. In conclusion, 5-aza-dC-modified microRNA profiles and identification of the microRNA's targets during the morula to blastocyst stage in mice provide information that helps us to explore the relationship between fertility, microRNA regulation and epigenetic intervention.

原文英語
文章編號gar072
頁(從 - 到)184-193
頁數10
期刊Molecular Human Reproduction
18
發行號4
DOIs
出版狀態已發佈 - 四月 2012

指紋

Morula
Blastocyst
DNA Methylation
MicroRNAs
decitabine
Epigenomics
Embryonic Structures
Genomic Imprinting
Methyltransferases
Gene Silencing
Fertility
Real-Time Polymerase Chain Reaction
Software
Databases
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Embryology
  • Cell Biology
  • Genetics
  • Developmental Biology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

引用此文

MicroRNA regulation via DNA methylation during the morula to blastocyst transition in mice. / Lee, Yee Ming; Chen, Huei Wen; Maurya, Pawan Kumar; Su, Ching Mao; Tzeng, Chii Ruey.

於: Molecular Human Reproduction, 卷 18, 編號 4, gar072, 04.2012, p. 184-193.

研究成果: 雜誌貢獻文章

Lee, Yee Ming ; Chen, Huei Wen ; Maurya, Pawan Kumar ; Su, Ching Mao ; Tzeng, Chii Ruey. / MicroRNA regulation via DNA methylation during the morula to blastocyst transition in mice. 於: Molecular Human Reproduction. 2012 ; 卷 18, 編號 4. 頁 184-193.
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abstract = "Epigenetic regulation is responsible for transcriptional silencing of genes and parental imprinting. This study addresses the question whether microRNAs (miRNAs) could be affected by DNA methylation during morula-blastocyst transition. Mouse embryos were treated with/without a DNA methyltransferase inhibitor (5-aza-2′-deoxycytidine, 5-aza-dC, 10 nM-5 μM). Changes of miRNAs were analyzed by quantitative real-time (Q-PCR)-based megaplex pre-amp microRNA assays. Development from morula to blastocyst in mice was inhibited by 5-aza-dC in a dose-dependent manner (10 nM-5 μM), with half of the embryos arrested at morula stage when treated with levels of 5-aza-dC as low as 50 nM. In total, 48 down-regulated microRNAs and 17 up-regulated microRNAs (≥5-fold changes) were identified after 5-aza-dC treatment, including let-7e, mir-20a, mir-21, mir-34b, mir-128b and mir-452. Their predicted targets were selected based on software analysis, published databases and further confirmed by Q-PCR. At least eight targets, including dnmt3a, jagged 1, sp1, edg2, abcg4, numa1, tmsb10 and csf1r were confirmed. In conclusion, 5-aza-dC-modified microRNA profiles and identification of the microRNA's targets during the morula to blastocyst stage in mice provide information that helps us to explore the relationship between fertility, microRNA regulation and epigenetic intervention.",
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