6 引文 (Scopus)

摘要

Toxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tunable manner. Therefore, in the current study, argon micro-plasma was introduced as a neutral energy source for crosslinking in fabrication of the desired gelatin-graphene oxide (gel-GO) nanocomposite hydrogel scaffolds. Argon microplasma was used to treat purified gelatin (8% w/v) containing 0.1~1 wt% of high-functionality nano-graphene oxide (GO). Optimized plasma conditions (2,500 V and 8.7 mA) for 15 min with a gas flow rate of 100 standard cm3/min was found to be most suitable for producing the gel-GO nanocomposite hydrogels. The developed hydrogel was characterized by the degree of cross-linking, FTIR spectroscopy, SEM, confocal microscopy, swelling behavior, contact angle measurement, and rheology. The cell viability was examined by an MTT assay and a live/dead assay. The pore size of the hydrogel was found to be 287 ± 27 mm with a contact angle of 78° ± 3.7°. Rheological data revealed improved storage as well as a loss modulus of up to 50% with tunable viscoelasticity, gel strength, and mechanical properties at 37 °C temperature in the microplasma-treated groups. The swelling behavior demonstrated a better water-holding capacity of the gel-GO hydrogels for cell growth and proliferation. Results of the MTT assay, microscopy, and live/dead assay exhibited better cell viability at 1% (w/w) of high-functionality GO in gelatin. The highlight of the present study is the first successful attempt of microplasmaassisted gelatin-GO nano composite hydrogel fabrication that offers great promise and optimism for further biomedical tissue engineering applications.
原文英語
頁(從 - 到)e3498
期刊PeerJ
2017
發行號6
DOIs
出版狀態已發佈 - 2017

指紋

nanocomposites
Graphite
Hydrogel
hydrocolloids
Gelatin
gelatin
Oxides
Fabrication
synthesis
crosslinking
Assays
Composite materials
Nanocomposites
Hydrogels
Argon
tissue engineering
Tissue Engineering
contact angle
Tissue engineering
assays

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

引用此文

Microplasma-assisted hydrogel fabrication : A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application. / Satapathy, Mantosh Kumar; Chiang, Wei Hung; Chuang, Er Yuan; Chen, Chih Hwa; Liao, Jia Liang; Huang, Huin Ning.

於: PeerJ, 卷 2017, 編號 6, 2017, p. e3498.

研究成果: 雜誌貢獻文章

Satapathy, Mantosh Kumar ; Chiang, Wei Hung ; Chuang, Er Yuan ; Chen, Chih Hwa ; Liao, Jia Liang ; Huang, Huin Ning. / Microplasma-assisted hydrogel fabrication : A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application. 於: PeerJ. 2017 ; 卷 2017, 編號 6. 頁 e3498.
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abstract = "Toxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tunable manner. Therefore, in the current study, argon micro-plasma was introduced as a neutral energy source for crosslinking in fabrication of the desired gelatin-graphene oxide (gel-GO) nanocomposite hydrogel scaffolds. Argon microplasma was used to treat purified gelatin (8{\%} w/v) containing 0.1~1 wt{\%} of high-functionality nano-graphene oxide (GO). Optimized plasma conditions (2,500 V and 8.7 mA) for 15 min with a gas flow rate of 100 standard cm3/min was found to be most suitable for producing the gel-GO nanocomposite hydrogels. The developed hydrogel was characterized by the degree of cross-linking, FTIR spectroscopy, SEM, confocal microscopy, swelling behavior, contact angle measurement, and rheology. The cell viability was examined by an MTT assay and a live/dead assay. The pore size of the hydrogel was found to be 287 ± 27 mm with a contact angle of 78° ± 3.7°. Rheological data revealed improved storage as well as a loss modulus of up to 50{\%} with tunable viscoelasticity, gel strength, and mechanical properties at 37 °C temperature in the microplasma-treated groups. The swelling behavior demonstrated a better water-holding capacity of the gel-GO hydrogels for cell growth and proliferation. Results of the MTT assay, microscopy, and live/dead assay exhibited better cell viability at 1{\%} (w/w) of high-functionality GO in gelatin. The highlight of the present study is the first successful attempt of microplasmaassisted gelatin-GO nano composite hydrogel fabrication that offers great promise and optimism for further biomedical tissue engineering applications.",
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AU - Chen, Chih Hwa

AU - Liao, Jia Liang

AU - Huang, Huin Ning

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KW - Graphene oxide

KW - Hydrogel

KW - Tissue engineering

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