Microglia-derived glial cell line-derived neurotrophic factor could protect Sprague-Dawley rat astrocyte from in vitro ischemia-induced damage

Gilbert A. Lee, Chi Hsin Lin, Hang Hsiang Jiang, Hsin Ju Chao, Chieh Liang Wu, Chi Mei Hsueh

研究成果: 雜誌貢獻文章同行評審

24 引文 斯高帕斯(Scopus)

摘要

The interrelationship between microglia and astrocytes in cerebral ischemia was determined in vitro by adding in vitro ischemia-induced supernatant from microglia into astrocytes under the same conditions (glucose-, oxygen- and serum-free). The involvement of glial cell line-derived neurotrophic factor (GDNF) was further investigated by immunoblocking assay and Western blot analysis. Results showed that microglia-derived supernatant protected against in vitro ischemia-induced damage of astrocytes and this protection was pre-blocked by anti-GDNF but not normal rabbit serum. In addition, in vitro ischemia appeared to induce the expression of GDNF in microglia. These results indicate that microglia-derived protection on astrocytes during in vitro ischemia is GDNF-dependent.
原文英語
頁(從 - 到)111-114
頁數4
期刊Neuroscience Letters
356
發行號2
DOIs
出版狀態已發佈 - 二月 12 2004

ASJC Scopus subject areas

  • Neuroscience(all)

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