Microcalorimetric studies on the physical stability of poly-ethylene glycol-grafted liposome

Der Zen Liu, Yi Ling Hsieh, Sheng Yen Chang, Wen Yih Chen

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)

摘要

Liposomes were prepared from mixtures of egg-PC, cholesterols and distearoyl-phosphatidyl-ethanolamine covalently attached poly-ethylene glycol (PEG) with a molecular of weight 2000 (DSPE-PEG2000). In this work examines how PEG2000-grafted lipids affect the surface properties of the egg phosphatidylcholine (egg-PC) liposomal bilayer membrane through zeta potential and interaction potential measurements using microcalorimetry. Experimental results demonstrate that the absolute value of the zeta potential of PEG2000-grafted PC liposomes decreased from -19 to -8 mV when increasing DSPE-PEG2000 from 0 to 7 mol fraction, and the repulsive interaction potential of PEG2000-grafted PC liposomes decreased compared with those liposomes without PEG-grafting. However, the phenomenon of fusion between the liposomes incorporated with PEG-grafted PC was reduced. In brief, this result of fusion is contrary to the expectation of the interaction potential measurement; therefore, we believe that the steric hindrance of the grafted PEG2000 molecules on the liposomal surface contribute to a major imposition on the approach between liposomal surface and the formation of inverted micelles which are suggested as the necessary steps of liposome fusion.

原文英語
頁(從 - 到)227-234
頁數8
期刊Colloids and Surfaces A: Physicochemical and Engineering Aspects
212
發行號2-3
DOIs
出版狀態已發佈 - 一月 23 2003

ASJC Scopus subject areas

  • 膠體和表面化學
  • 物理與理論化學

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