Methylomic analysis identifies frequent DNA methylation of Zinc Finger protein 582 (ZNF582) in cervical neoplasms

Rui Lan Huang, Cheng Chang Chang, Po Hsuan Su, Yu Chih Chen, Yu Ping Liao, Hui Chen Wang, Yi Te Yo, Tai Kuang Chao, Hsuan Cheng Huang, Ching Yu Lin, Tang Yuan Chu, Hung Cheng Lai

研究成果: 雜誌貢獻文章

51 引文 (Scopus)

摘要

Background: Despite of the trend that the application of DNA methylation as a biomarker for cancer detection is promising, clinically applicable genes are few. Therefore, we looked for novel hypermethylated genes for cervical cancer screening. Methods and Findings: At the discovery phase, we analyzed the methylation profiles of human cervical carcinomas and normal cervixes by methylated DNA immunoprecipitation coupled to promoter tiling arrays (MeDIP-on-chip). Methylation-specific PCR (MSP), quantitative MSP and bisulfite sequencing were used to verify the methylation status in cancer tissues and cervical scrapings from patients with different severities. Immunohistochemical staining of a cervical tissue microarray was used to confirm protein expression. We narrowed to three candidate genes: DBC1, PDE8B, and ZNF582; their methylation frequencies in tumors were 93%, 29%, and 100%, respectively. At the pre-validation phase, the methylation frequency of DBC1 and ZNF582 in cervical scraping correlated significantly with disease severity in an independent cohort (n = 330, both P
原文英語
文章編號e41060
期刊PLoS One
7
發行號7
DOIs
出版狀態已發佈 - 七月 16 2012

指紋

uterine cervical neoplasms
Methylation
zinc finger motif
Zinc Fingers
DNA methylation
DNA Methylation
Uterine Cervical Neoplasms
methylation
Zinc
Proteins
proteins
Genes
neoplasms
Tissue
bisulfites
Polymerase Chain Reaction
genes
cervix
Microarrays
Tumor Biomarkers

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

引用此文

Methylomic analysis identifies frequent DNA methylation of Zinc Finger protein 582 (ZNF582) in cervical neoplasms. / Huang, Rui Lan; Chang, Cheng Chang; Su, Po Hsuan; Chen, Yu Chih; Liao, Yu Ping; Wang, Hui Chen; Yo, Yi Te; Chao, Tai Kuang; Huang, Hsuan Cheng; Lin, Ching Yu; Chu, Tang Yuan; Lai, Hung Cheng.

於: PLoS One, 卷 7, 編號 7, e41060, 16.07.2012.

研究成果: 雜誌貢獻文章

Huang, Rui Lan ; Chang, Cheng Chang ; Su, Po Hsuan ; Chen, Yu Chih ; Liao, Yu Ping ; Wang, Hui Chen ; Yo, Yi Te ; Chao, Tai Kuang ; Huang, Hsuan Cheng ; Lin, Ching Yu ; Chu, Tang Yuan ; Lai, Hung Cheng. / Methylomic analysis identifies frequent DNA methylation of Zinc Finger protein 582 (ZNF582) in cervical neoplasms. 於: PLoS One. 2012 ; 卷 7, 編號 7.
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abstract = "Background: Despite of the trend that the application of DNA methylation as a biomarker for cancer detection is promising, clinically applicable genes are few. Therefore, we looked for novel hypermethylated genes for cervical cancer screening. Methods and Findings: At the discovery phase, we analyzed the methylation profiles of human cervical carcinomas and normal cervixes by methylated DNA immunoprecipitation coupled to promoter tiling arrays (MeDIP-on-chip). Methylation-specific PCR (MSP), quantitative MSP and bisulfite sequencing were used to verify the methylation status in cancer tissues and cervical scrapings from patients with different severities. Immunohistochemical staining of a cervical tissue microarray was used to confirm protein expression. We narrowed to three candidate genes: DBC1, PDE8B, and ZNF582; their methylation frequencies in tumors were 93{\%}, 29{\%}, and 100{\%}, respectively. At the pre-validation phase, the methylation frequency of DBC1 and ZNF582 in cervical scraping correlated significantly with disease severity in an independent cohort (n = 330, both P",
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AU - Chen, Yu Chih

AU - Liao, Yu Ping

AU - Wang, Hui Chen

AU - Yo, Yi Te

AU - Chao, Tai Kuang

AU - Huang, Hsuan Cheng

AU - Lin, Ching Yu

AU - Chu, Tang Yuan

AU - Lai, Hung Cheng

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