Methylation of the Tumor Suppressor Genes HIC1 and RassF1A Clusters Independently From the Methylation of Polycomb Target Genes in Colon Cancer

Hong Chang Chen, Hsuan Yuan Huang, Yao Li Chen, Kuan Der Lee, Yi Ru Chu, Ping Yi Lin, Chia Chen Hsu, Pei Yi Chu, Tim H.M. Huang, Shu Huei Hsiao, Yu Wei Leu

研究成果: 雜誌貢獻文章

2 引文 斯高帕斯(Scopus)

摘要

Background: Methylation changes within tumor suppressor (TS) genes or polycomb group target (PcG) genes alter cell fates. Chromatin associated with PcG targets is bivalent in stem cells, while TS genes are not normally bivalent. PcG target methylation changes have been identified in tumor stem cells, and abnormal methylation is found in TS genes in cancers. If the epigenetic states of genes influence DNA methylation, then methylation of PcG targets and TS genes may evolve differently during cancer development. More importantly, methylation changes may be part of a sequence in tumorigenesis. Methods: Chromatin and methylation states of 4 PcG targets and 2 TS genes were determined in colon cancer cells. The methylation states were also detected in 100 pairs of colon cancer samples. Principle component analysis (PCA) was used to reveal whether TS methylation or PcG methylation was the main methylation change associated with colon cancers. Results: Chromatin and methylation states differ in colon cancer cell lines. The methylation states within PcG targets clustered independently from the methylation states in TS genes, a finding we previously reported in liver cancers. PCA in colon cancers revealed the strongest association with methylation changes in 2 TS genes, HIC1 and RassF1A. Loss of HIC1 methylation correlated with decreased tumor migration. Conclusions: PcG and TS methylation states cluster independently from each other. The deduced principle component correlated better with TS methylation than PcG methylation in colon cancer. Abnormal methylation changes may represent a sequential biomarker profile to identify developing colon cancer.
原文英語
頁(從 - 到)578-585
頁數8
期刊Annals of Surgical Oncology
24
發行號2
DOIs
出版狀態已發佈 - 二月 1 2017
對外發佈Yes

    指紋

ASJC Scopus subject areas

  • Surgery
  • Oncology

引用此

Chen, H. C., Huang, H. Y., Chen, Y. L., Lee, K. D., Chu, Y. R., Lin, P. Y., Hsu, C. C., Chu, P. Y., Huang, T. H. M., Hsiao, S. H., & Leu, Y. W. (2017). Methylation of the Tumor Suppressor Genes HIC1 and RassF1A Clusters Independently From the Methylation of Polycomb Target Genes in Colon Cancer. Annals of Surgical Oncology, 24(2), 578-585. https://doi.org/10.1245/s10434-015-5024-z