Methionine oxidation in albumin by fine haze particulate matter: An in vitro and in vivo study

Kang Yun Lee, Chris Kong Chu Wong, Kai Jen Chuang, Mauo Ying Bien, Jun Ji Cao, Yong Ming Han, Linwei Tian, Chih Cheng Chang, Po Hao Feng, Kin Fai Ho, Hsiao Chi Chuang

研究成果: 雜誌貢獻文章

17 引文 斯高帕斯(Scopus)

摘要

The potential effects of inhaled fine particulate matter (PM2.5), found in haze episodes, on the oxidation of the proteins in the lungs are not well understood. We investigated the effects of PM2.5 from haze episodes on protein oxidation. PM2.5 was collected from the air pollution in Beijing (BJ), Xian (XA), Xiamen (XM) and Hong Kong (HK) during a period of intensive haze episodes. The chemical characteristics of these samples and their effects on albumin oxidation were investigated. The levels of PM2.5 in BJ and XA were 4-6 times higher than in XM and HK. The concentrations of the polycyclic aromatic hydrocarbons (PAHs) components of the PM2.5 from BJ and XA were 10 times higher than those found in XM and HK. The haze PM2.5 increased oxidative stress. Addition of PM2.5 samples collected from haze episodes to albumin in vitro resulted in oxidation of methionine moieties; nasal instillation of PM2.5 suspensions in mice resulted in oxidation of methionine in the albumin in the bronchoalveolar lavage fluid. The methionine moieties participate in peptide chain crosslinking, and methionine oxidation in the albumin could be attributed to the PAH compounds. Our findings may be helpful in explaining the potential respiratory effects during haze episodes.

原文英語
頁(從 - 到)384-391
頁數8
期刊Journal of Hazardous Materials
274
DOIs
出版狀態已發佈 - 六月 15 2014

    指紋

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering
  • Medicine(all)

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