Methanol-soluble, β-elimination products from preparations of alginic acid hydroxamate exhibited DPPH scavenging and angiotensin converting enzyme inhibitory activities

Yuh H. Liu, Mao T. Chuang, Wen C. Hou

研究成果: 雜誌貢獻文章同行評審

6 引文 斯高帕斯(Scopus)

摘要

The methanol-soluble sugar hydroxamic acid derivatives obtained during the preparation of alginic acid hydroxamates (AAH) were separated by BioSil-ODS HPLC column (10x250 mm) in acetonitrite: 0.05% trifluoroacetic acid, 10:90 (V/V). The absorbance at 235 nm was set for monitoring β-elimination products. Each fraction was collected and assayed for hydroxamic acid contents, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and inhibitory activity against angiotensin converting enzyme (ACE). The fraction with higher hydroxamic acid content was further separated by Sephadex G-15 column. Fractions containing both ACE inhibitory activity and DPPH scavenging activity overlapped with fractions containing high levels of hydroxamic acid derivatives. The chromatogram showed little tailing when judged by hydroxamic-acid content but significant tailing when judged by biological activity. Each fraction from above was further separated by silica-TLC in acetonitrite: distilled water, 95:5 (V/V). It was found that a methanol soluble fraction prepared from alginic acid hydroxamates (AAH) exhibited DPPH scavenging activity.

原文英語
頁(從 - 到)141-146
頁數6
期刊Botanical Studies
48
發行號2
出版狀態已發佈 - 四月 2007

ASJC Scopus subject areas

  • 植物科學

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