Metabolomics investigation of exercise-modulated changes in metabolism in rat liver after exhaustive and endurance exercises

Chi Chang Huang, Wan Teng Lin, Feng Lin Hsu, Pi Wen Tsai, Chia Chung Hou

研究成果: 雜誌貢獻文章同行評審

59 引文 斯高帕斯(Scopus)

摘要

Exhaustive exercise and endurance exercise training modify the physiological status of the body differently. The present study aimed to evaluate the alteration in biochemical composition with exhaustive and endurance exercises in rats using metabolomics strategy. The metabolite profile of liver tissue was investigated on gas chromatography-mass spectrometry (GC-MS). Data further underwent partial least-squares-discriminant analysis (PLS-DA) to compare the effects on metabolites in sedentary control, exhaustively exercised and endurance trained rats. GC-MS detected 115 highly reproducible peaks in chromatograms from individual liver tissue extracts, and we identified 55 of them. The three groups showed significant differences in metabolic profile. Changes in liver metabolism involved metabolites such as amino acids, fatty acids, organic acids, and carbohydrates. Endurance training elevated the greater rate of tricarboxylic acid cycle and antioxidant activity, and exhaustive exercise led to accumulated urea markers and an inflammation response in liver. In addition, GC-MS-based metabolomic analysis is a promising tool to investigate a pathological status with different exercise programs.
原文英語
頁(從 - 到)557-566
頁數10
期刊European Journal of Applied Physiology
108
發行號3
DOIs
出版狀態已發佈 - 2月 2010

ASJC Scopus subject areas

  • 公共衛生、環境和職業健康
  • 骨科和運動醫學
  • 生理學(醫學)

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