Metabolomics characterization of energy metabolism reveals glycogen accumulation in gut-microbiota-lacking mice

Hsiao Li Chuang, Yen Te Huang, Chien Chao Chiu, Chia Ding Liao, Feng Lin Hsu, Chi Chang Huang, Chia Chung Hou

研究成果: 雜誌貢獻文章

19 引文 (Scopus)

摘要

Microbiota in the gut are considered an important environmental factor associated with host metabolism and physiology. Although gut microbiota are known to contribute to hepatic lipogenesis and fat storage, little is known about how the condition influences the deposition of glycogen in the liver. To better understand and characterize the host energy metabolism in guts lacking microbiota, we compared the liver metabolome of specific pathogen-free and germ-free mice by gas chromatography-mass spectrometry combined with partial least-squares discriminant analysis. We identified 30 of 52 highly reproducible peaks in chromatograms of liver tissue extracts from the two groups of mice. The two groups showed significant differences in metabolic profile. Changes in liver metabolism involved metabolites such as amino acids, fatty acids, organic acids and carbohydrates. The metabolic profile of germ-free mice suggests that they synthesize glycogen and accumulate it in the liver through gluconeogenesis and glycogenesis. Our findings shed light on a new perspective of the role of gut microbiota in energy metabolism and will be useful to help study probiotics, obesity and metabolic diseases.
原文英語
頁(從 - 到)752-758
頁數7
期刊Journal of Nutritional Biochemistry
23
發行號7
DOIs
出版狀態已發佈 - 七月 2012

指紋

Metabolomics
Glycogen
Liver
Energy Metabolism
Metabolome
Metabolism
Specific Pathogen-Free Organisms
Liver Extracts
Lipogenesis
Liver Glycogen
Tissue Extracts
Gluconeogenesis
Metabolic Diseases
Probiotics
Discriminant Analysis
Least-Squares Analysis
Gas Chromatography-Mass Spectrometry
Organic acids
Physiology
Discriminant analysis

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

引用此文

Metabolomics characterization of energy metabolism reveals glycogen accumulation in gut-microbiota-lacking mice. / Chuang, Hsiao Li; Huang, Yen Te; Chiu, Chien Chao; Liao, Chia Ding; Hsu, Feng Lin; Huang, Chi Chang; Hou, Chia Chung.

於: Journal of Nutritional Biochemistry, 卷 23, 編號 7, 07.2012, p. 752-758.

研究成果: 雜誌貢獻文章

Chuang, Hsiao Li ; Huang, Yen Te ; Chiu, Chien Chao ; Liao, Chia Ding ; Hsu, Feng Lin ; Huang, Chi Chang ; Hou, Chia Chung. / Metabolomics characterization of energy metabolism reveals glycogen accumulation in gut-microbiota-lacking mice. 於: Journal of Nutritional Biochemistry. 2012 ; 卷 23, 編號 7. 頁 752-758.
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abstract = "Microbiota in the gut are considered an important environmental factor associated with host metabolism and physiology. Although gut microbiota are known to contribute to hepatic lipogenesis and fat storage, little is known about how the condition influences the deposition of glycogen in the liver. To better understand and characterize the host energy metabolism in guts lacking microbiota, we compared the liver metabolome of specific pathogen-free and germ-free mice by gas chromatography-mass spectrometry combined with partial least-squares discriminant analysis. We identified 30 of 52 highly reproducible peaks in chromatograms of liver tissue extracts from the two groups of mice. The two groups showed significant differences in metabolic profile. Changes in liver metabolism involved metabolites such as amino acids, fatty acids, organic acids and carbohydrates. The metabolic profile of germ-free mice suggests that they synthesize glycogen and accumulate it in the liver through gluconeogenesis and glycogenesis. Our findings shed light on a new perspective of the role of gut microbiota in energy metabolism and will be useful to help study probiotics, obesity and metabolic diseases.",
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