Metabolomic characterization of rhubarb species by capillary electrophoresis and ultra-high-pressure liquid chromatography

Yufeng Jane Tseng, Chun Ting Kuo, San Yuan Wang, Hsiao Wei Liao, Guan Yuan Chen, Yuan Ling Ku, Wei Cheng Shao, Ching Hua Kuo

研究成果: 雜誌貢獻文章

7 引文 (Scopus)

摘要

This study developed CE and ultra-high-pressure LC (UHPLC) methods coupled with UV detectors to characterize the metabolomic profiles of different rhubarb species. The optimal CE conditions used a BGE with 15 mM sodium tetraborate, 15 mM sodium dihydrogen phosphate monohydrate, 30 mM sodium deoxycholate, and 30% ACN v/v at pH 8.3. The optimal UHPLC conditions used a mobile phase composed of 0.05% phosphate buffer and ACN with gradient elution. The gradient profile increased linearly from 10 to 21% ACN within the first 25 min, then increased to 33% ACN for the next 10 min. It took another 5 min to reach the 65% ACN, then for the next 5 min, it stayed unchanged. Sixteen samples of Rheum officinale and Rheum tanguticum collected from various locations were analyzed by CE and UHPLC methods. The metabolite profiles of CE were aligned and baseline corrected before chemometric analysis. Metabolomic signatures of rhubarb species from CE and UHPLC were clustered using principle component analysis and distance-based redundancy analysis; the clusters were not only able to discriminate different species but also different cultivation regions. Similarity measurements were performed by calculating the correlation coefficient of each sample with the authentic samples. Hybrid rhizome was clearly identified through similarity measurement of UHPLC metabolite profile and later confirmed by gene sequencing. The present study demonstrated that CE and UHPLC are efficient and effective tools to identify and authenticate herbs even coupled with simple detectors.
原文英語
頁(從 - 到)2918-2927
頁數10
期刊Electrophoresis
34
發行號19
DOIs
出版狀態已發佈 - 十月 1 2013
對外發佈Yes

指紋

High pressure liquid chromatography
Rheum
Capillary electrophoresis
Metabolomics
Capillary Electrophoresis
High Pressure Liquid Chromatography
Pressure
Metabolites
Ultraviolet detectors
Rhizome
Deoxycholic Acid
Redundancy
Buffers
Genes
Phosphates
3-hydroxy-5-estrane-17-carbonitrile
Detectors

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

引用此文

Metabolomic characterization of rhubarb species by capillary electrophoresis and ultra-high-pressure liquid chromatography. / Tseng, Yufeng Jane; Kuo, Chun Ting; Wang, San Yuan; Liao, Hsiao Wei; Chen, Guan Yuan; Ku, Yuan Ling; Shao, Wei Cheng; Kuo, Ching Hua.

於: Electrophoresis, 卷 34, 編號 19, 01.10.2013, p. 2918-2927.

研究成果: 雜誌貢獻文章

Tseng, Yufeng Jane ; Kuo, Chun Ting ; Wang, San Yuan ; Liao, Hsiao Wei ; Chen, Guan Yuan ; Ku, Yuan Ling ; Shao, Wei Cheng ; Kuo, Ching Hua. / Metabolomic characterization of rhubarb species by capillary electrophoresis and ultra-high-pressure liquid chromatography. 於: Electrophoresis. 2013 ; 卷 34, 編號 19. 頁 2918-2927.
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abstract = "This study developed CE and ultra-high-pressure LC (UHPLC) methods coupled with UV detectors to characterize the metabolomic profiles of different rhubarb species. The optimal CE conditions used a BGE with 15 mM sodium tetraborate, 15 mM sodium dihydrogen phosphate monohydrate, 30 mM sodium deoxycholate, and 30{\%} ACN v/v at pH 8.3. The optimal UHPLC conditions used a mobile phase composed of 0.05{\%} phosphate buffer and ACN with gradient elution. The gradient profile increased linearly from 10 to 21{\%} ACN within the first 25 min, then increased to 33{\%} ACN for the next 10 min. It took another 5 min to reach the 65{\%} ACN, then for the next 5 min, it stayed unchanged. Sixteen samples of Rheum officinale and Rheum tanguticum collected from various locations were analyzed by CE and UHPLC methods. The metabolite profiles of CE were aligned and baseline corrected before chemometric analysis. Metabolomic signatures of rhubarb species from CE and UHPLC were clustered using principle component analysis and distance-based redundancy analysis; the clusters were not only able to discriminate different species but also different cultivation regions. Similarity measurements were performed by calculating the correlation coefficient of each sample with the authentic samples. Hybrid rhizome was clearly identified through similarity measurement of UHPLC metabolite profile and later confirmed by gene sequencing. The present study demonstrated that CE and UHPLC are efficient and effective tools to identify and authenticate herbs even coupled with simple detectors.",
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AU - Liao, Hsiao Wei

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AU - Ku, Yuan Ling

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N2 - This study developed CE and ultra-high-pressure LC (UHPLC) methods coupled with UV detectors to characterize the metabolomic profiles of different rhubarb species. The optimal CE conditions used a BGE with 15 mM sodium tetraborate, 15 mM sodium dihydrogen phosphate monohydrate, 30 mM sodium deoxycholate, and 30% ACN v/v at pH 8.3. The optimal UHPLC conditions used a mobile phase composed of 0.05% phosphate buffer and ACN with gradient elution. The gradient profile increased linearly from 10 to 21% ACN within the first 25 min, then increased to 33% ACN for the next 10 min. It took another 5 min to reach the 65% ACN, then for the next 5 min, it stayed unchanged. Sixteen samples of Rheum officinale and Rheum tanguticum collected from various locations were analyzed by CE and UHPLC methods. The metabolite profiles of CE were aligned and baseline corrected before chemometric analysis. Metabolomic signatures of rhubarb species from CE and UHPLC were clustered using principle component analysis and distance-based redundancy analysis; the clusters were not only able to discriminate different species but also different cultivation regions. Similarity measurements were performed by calculating the correlation coefficient of each sample with the authentic samples. Hybrid rhizome was clearly identified through similarity measurement of UHPLC metabolite profile and later confirmed by gene sequencing. The present study demonstrated that CE and UHPLC are efficient and effective tools to identify and authenticate herbs even coupled with simple detectors.

AB - This study developed CE and ultra-high-pressure LC (UHPLC) methods coupled with UV detectors to characterize the metabolomic profiles of different rhubarb species. The optimal CE conditions used a BGE with 15 mM sodium tetraborate, 15 mM sodium dihydrogen phosphate monohydrate, 30 mM sodium deoxycholate, and 30% ACN v/v at pH 8.3. The optimal UHPLC conditions used a mobile phase composed of 0.05% phosphate buffer and ACN with gradient elution. The gradient profile increased linearly from 10 to 21% ACN within the first 25 min, then increased to 33% ACN for the next 10 min. It took another 5 min to reach the 65% ACN, then for the next 5 min, it stayed unchanged. Sixteen samples of Rheum officinale and Rheum tanguticum collected from various locations were analyzed by CE and UHPLC methods. The metabolite profiles of CE were aligned and baseline corrected before chemometric analysis. Metabolomic signatures of rhubarb species from CE and UHPLC were clustered using principle component analysis and distance-based redundancy analysis; the clusters were not only able to discriminate different species but also different cultivation regions. Similarity measurements were performed by calculating the correlation coefficient of each sample with the authentic samples. Hybrid rhizome was clearly identified through similarity measurement of UHPLC metabolite profile and later confirmed by gene sequencing. The present study demonstrated that CE and UHPLC are efficient and effective tools to identify and authenticate herbs even coupled with simple detectors.

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