Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells

Hon Man Liu, Si Han Wu, Chen Wen Lu, Ming Yao, Jong Kai Hsiao, Yann Hung, Yu Shen Lin, Chung Yuan Mou, Chung Shi Yang, Dong Ming Huang, Yao Chang Chen

研究成果: 雜誌貢獻文章

110 引文 斯高帕斯(Scopus)

摘要

Tumblerlike magnetic/fluorescein isothiocyanate (FITC)-labeled mesoporous silica nanoparticles, Mag-Dye@MSNs, have been developed, which are composed of silica-coated core-shell superparamagnetic iron oxide (SPICK&SiO 2) nanoparticles co-condensed with FITC-incorporated mesoporous silica. Mag-Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and in vivo, as manifested by using a clinical 1.5-T MRI system with requirements of simultaneous low incubation dosage of iron, low detection cell numbers, and short incubation time. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes, which can still be readily detected by MRI. Moreover, a higher MRI signal intensity decrease is observed in Mag-Dye@MSN-treated cells than in SPIO@SiO2-treated cells. This is the first report that MCM-41-type MSNs are advantageous to cellular uptake, as manifested by a higher labeling efficiency of Mag-Dye@MSNs than SPIO@SiO2.

原文英語
頁(從 - 到)619-626
頁數8
期刊Small
4
發行號5
DOIs
出版狀態已發佈 - 五月 2008
對外發佈Yes

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

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    Liu, H. M., Wu, S. H., Lu, C. W., Yao, M., Hsiao, J. K., Hung, Y., Lin, Y. S., Mou, C. Y., Yang, C. S., Huang, D. M., & Chen, Y. C. (2008). Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells. Small, 4(5), 619-626. https://doi.org/10.1002/smll.200700493