Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

Lauren R. Clements; Peng Yuan Wang; Frances Harding; Wei Bor Tsai; Helmut Thissen; Nicolas H. Voelcker

doi:10.1002/pssa.201000320

Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

Lauren R. Clements, Peng Yuan Wang, Frances Harding, Wei Bor Tsai, Helmut Thissen, Nicolas H. Voelcker

研究成果: 雜誌貢獻 › 文章 › 同行評審

29 引文斯高帕斯（Scopus）

摘要

Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.

原文	英語
頁（從 - 到）	1440-1445
頁數	6
期刊	Physica Status Solidi (A) Applications and Materials Science
卷	208
發行號	6
DOIs	https://doi.org/10.1002/pssa.201000320
出版狀態	已發佈 - 6月 2011
對外發佈	是

ASJC Scopus subject areas

電氣與電子工程
電子、光磁材料
材料化學
凝聚態物理學
表面和介面
表面、塗料和薄膜

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10.1002/pssa.201000320

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Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting. / Clements, Lauren R.; Wang, Peng Yuan; Harding, Frances; Tsai, Wei Bor; Thissen, Helmut; Voelcker, Nicolas H.

於: Physica Status Solidi (A) Applications and Materials Science, 卷 208, 編號 6, 06.2011, p. 1440-1445.

研究成果: 雜誌貢獻 › 文章 › 同行評審

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abstract = "Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.",

keywords = "biomaterial, electrochemistry, gradient surfaces, porous silicon, surface chemistry",

author = "Clements, {Lauren R.} and Wang, {Peng Yuan} and Frances Harding and Tsai, {Wei Bor} and Helmut Thissen and Voelcker, {Nicolas H.}",

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AU - Harding, Frances

AU - Tsai, Wei Bor

AU - Thissen, Helmut

AU - Voelcker, Nicolas H.

PY - 2011/6

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AB - Chemical gradients of ethyl-6-bromohexanoate (EBH) were generated over porous silicon (pSi) substrates via electrochemical attachment. Immobilised ester moieties were hydrolysed and activated to produce a gradient of functional carboxylic acid groups. After subsequent immobilisation of cyclic RGD (cRGD), the surfaces were used to screen the extent of rat mesenchymal stem cell (MSC) attachment. Mapping of surface chemistry was carried out by means of infrared microscopy and X-ray photoelectron spectroscopy (XPS). MSC culture studies showed that short-term cell attachment responded to the cRGD density present on the gradient surface.

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KW - electrochemistry

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KW - surface chemistry

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Mesenchymal stem cell attachment to peptide density gradients on porous silicon generated by electrografting

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