Mechanistic insights to catalysis by a zinc-dependent bi-functional nuclease from Arabidopsis thaliana

Tsung Lin Chou, Tzu Ping Ko, Chia Yun Ko, Tsai Yun Lin, Rey Ting Guo, Tsung Fu Yu, Hsiu Chien Chan, Jei Fu Shaw, Andrew H.J. Wang

研究成果: 雜誌貢獻文章同行評審

4 引文 斯高帕斯(Scopus)

摘要

The bi-functional nuclease AtBFN2 from Arabidopsis thaliana (EC 3.1.30.1) depends on zinc ion for cleaving single stranded DNA and RNA to yield 5'-nucleotides. It is a glycoprotein that participates in plant development and differentiation. The crystal structure of AtBFN2 shows a bound sulfate ion in the active site, at the center of the tri-nuclear cluster of zinc ions. The protein folds into a mostly α-helical structure with five short β-strands and contains four disulfide bonds. The zinc ions are coordinated to the side chains of three Asp and five His residues, two backbone atoms of Trp1, the sulfate ion, and a water molecule. An adenine base is bound adjacent to the active site and stacks with Tyr59. The core sugar residues attached to the three N-glycosylation sites of Asn91, Asn110 and Asn184 are also observed. By comparison with the nuclease P1 structure (PDB ID: 1AK0), the AtBFN2-sulfate-adenine complex model suggests a similar catalytic mechanism, in which the reaction starts with in-line attack at the phosphate by a zinc-activated water molecule.

原文英語
頁(從 - 到)191-195
頁數5
期刊Biocatalysis and Agricultural Biotechnology
2
發行號3
DOIs
出版狀態已發佈 - 7月 2013
對外發佈

ASJC Scopus subject areas

  • 生物技術
  • 生物工程
  • 食品科學
  • 應用微生物與生物技術
  • 農學與作物科學

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