Mechanisms underlying the induction of vasorelaxation in rat thoracic aorta by sanguinarine

C. M. Hu, H. W. Cheng, Y. W. Cheng, J. J. Kang

研究成果: 雜誌貢獻文章同行評審

21 引文 斯高帕斯(Scopus)

摘要

In the present study, the effect of sanguinarine (SANG) on smooth muscle was investigated in thoracic aorta isolated from rats. SANG dose-dependently relaxed the phenylephrine (PE, 3 μM)-precontracted aorta; and the concentrations to produce 50% relaxation were 3.18 ± 0.37 and 3.42 ± 1.14 μM, respectively, in intact and denuded aorta. These results suggest that the relaxing effect of SANG was endothelium-independent. The total contraction induced by PE was inhibited in aorta pretreated with SANG at μM concentration. Both phasic and tonic contractions induced by PE were inhibited by SANG independently, which were further supported by the fact that inositol 1,4,5-trisphosphate (IP 3) formation and 45Ca 2+ influx induced by 3 μM PE in denuded aorta were inhibited by SANG concentration-dependently. In addition, the vasocontraction induced by high-K + was also inhibited by SANG, however, at higher concentrations. The inhibitory effects of SANG were reversed by dithiothreitol, a thiol reducing agent, implying that the oxidation of critical sulfhydryl groups on key molecules that regulate the smooth muscle contraction were involved. These data suggested that the inhibitory effects of SANG on PE-induced vasocontraction might involve the inhibition of IP 3 formation and blockade of calcium channel.
原文英語
頁(從 - 到)47-53
頁數7
期刊Japanese Journal of Pharmacology
85
發行號1
DOIs
出版狀態已發佈 - 2001
對外發佈Yes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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