Mechanisms involved in the antiplatelet activity of rutin, a glycoside of the flavonol quercetin, in human platelets

Joen Rong Sheu, George Hsiao, Po Hsiu Chou, Ming Yi Shen, Duen Suey Chou

研究成果: 雜誌貢獻文章

99 引文 斯高帕斯(Scopus)

摘要

The aim of this study was to systematically examine the inhibitory mechanisms of rutin, a well-known flavonoid in platelet aggregation. In this study, rutin concentration-dependently (250 and 290 μM) inhibited platelet aggregation in human platelets stimulated by agonists (i.e., collagen). Rutin (250 and 290 μM) did not significantly interfere with the binding of FITC-triflavin to the glycoprotein IIb/IIIa complex in human platelets. Rutin (250 and 290 μM) markedly inhibited intracellular Ca2+ mobilization and thromboxane A2 formation in human platelets stimulated by collagen. Rapid phosphorylation of a platelet protein of M r 47000 (P47), a marker of protein kinase C activation, was triggered by collagen (1 μg/mL). This phosphorylation was markedly inhibited by rutin (250 and 290 μM). On the other hand, rutin (250 and 290 μM) did not significantly increase the formations of cyclic AMP and nitric oxide/cyclic GMP in platelets. In conclusion, these results indicate that the antiplatelet activity of rutin may involve the following pathways: rutin inhibited the activation of phospholipase C, followed by inhibition of protein kinase C activity and thromboxane A2 formation, thereby leading to inhibition of the phosphorylation of P47 and intracellular Ca2+ mobilization, finally resulting in inhibition of platelet aggregation.
原文英語
頁(從 - 到)4414-4418
頁數5
期刊Journal of Agricultural and Food Chemistry
52
發行號14
DOIs
出版狀態已發佈 - 七月 14 2004

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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