Mechanical coupling of cytoskeletal elasticity and force generation is crucial for understanding the migrating nature of keloid fibroblasts

Hans I Chen Harn, Yang Kao Wang, Chao Kai Hsu, Yen Ting Ho, Yi Wei Huang, Wen Tai Chiu, Hsi Hui Lin, Chao Min Cheng, Ming Jer Tang

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    21 引文 斯高帕斯(Scopus)

    摘要

    One of the key features of keloid is its fibroblasts migrating beyond the original wound border. During migration, cells not only undergo molecular changes but also mechanical modulation. This process is led by actin filaments serving as the backbone of intra-cellular force and transduces external mechanical signal via focal adhesion complex into the cell. Here, we focus on determining the mechanical changes of actin filaments and the spatial distribution of forces in response to changing chemical stimulations and during cell migration. Atomic force microscopy and micropost array detector are used to determine and compare the magnitude and distribution of filament elasticity and force generation in fibroblasts and keloid fibroblasts. We found both filament elasticity and force generation show spatial distribution in a polarized and migrating cell. Such spatial distribution is disrupted when mechano-signalling is perturbed by focal adhesion kinase inhibitor and in keloid fibroblasts. The demonstration of keloid pathology at the nanoscale highlights the coupling of cytoskeletal function with physical characters at the subcellular level and provides new research directions for migration-related disease such as keloid.
    原文英語
    頁(從 - 到)579-584
    頁數6
    期刊Experimental Dermatology
    24
    發行號8
    DOIs
    出版狀態已發佈 - 八月 1 2015

    ASJC Scopus subject areas

    • Dermatology
    • Molecular Biology
    • Biochemistry
    • Medicine(all)

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