Measurement of intracellular ion dynamics with microfluorescent ratio imaging system

C. W. Lin, C. J. Yao, T. S. Ko, S. Y. Lin-Shiau

研究成果: 雜誌貢獻文章同行評審

4 引文 斯高帕斯(Scopus)

摘要

To study the ionic dynamics of spatial distribution and cellular signaling, we have setup a microfluorescent ratio imaging system for quantitative measurement of intracellular calcium and proton concentration with fluorescent indicator, Fura-2 and BCECF. Combined with cell culture techniques, this setup can measure responses from single cell and cell to cell interactions in time lapse mode. The in-homogeneous distribution of intracellular calcium ions under resting condition and after chemical stimulus can also be clearly seen and subjected to quantitative measurement. The results indicate that the resting level of calcium is around 90 nM before maturation (2 DIV.) while significantly raised to around 110 nM after maturation (7 DIV.) in cultured cerebellar granule neurons. Thapsigargin (Thsg) does not transient increase intracellular calcium level as reported in other cell preparations.
原文英語
頁(從 - 到)131-138
頁數8
期刊Biomedical Engineering - Applications, Basis and Communications
10
發行號3
出版狀態已發佈 - 六月 25 1998
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物工程

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