MCPIP1 ribonuclease exhibits broad-spectrum antiviral effects through viral RNA binding and degradation

Ren-Jye Lin, Hsu Ling Chien, Shyr Yi Lin, Bi Lan Chang, Han Pang Yu, Wei Chun Tang, Yi Ling Lin

研究成果: 雜誌貢獻文章同行評審

81 引文 斯高帕斯(Scopus)

摘要

Monocyte chemoattractant protein 1-induced protein 1 (MCPIP1), belonging to the MCPIP family with highly conserved CCCH-type zinc finger and Nedd4-BP1, YacP Nuclease domains, has been implicated in negative regulation of the cellular inflammatory responses. In this report, we demonstrate for the first time that this RNA-binding nuclease also targets viral RNA and possesses potent antiviral activities. Overexpression of the human MCPIP1, but not MCPIP2, MCPIP3 or MCPIP4, inhibited Japanese encephalitis virus (JEV) and dengue virus (DEN) replication. The functional analysis of MCPIP1 revealed that the activities of RNase, RNA binding and oligomerization, but not deubiqutinase, are required for its antiviral potential. Furthermore, infection of other positive-sense RNA viruses, such as sindbis virus and encephalomyocarditis virus, and negative-sense RNA virus, such as influenza virus, as well as DNA virus, such as adenovirus, can also be blocked by MCPIP1. Moreover, the endogenous MCPIP1 gene expression was induced by JEV and DEN infection, and knockdown of MCPIP1 expression enhanced the replication of JEV and DEN in human cells. Thus, MCPIP1 can act as a host innate defense via RNase activity for targeting and degrading viral RNA.
原文英語
頁(從 - 到)3314-3326
頁數13
期刊Nucleic Acids Research
41
發行號5
DOIs
出版狀態已發佈 - 三月 2013

ASJC Scopus subject areas

  • Genetics

指紋 深入研究「MCPIP1 ribonuclease exhibits broad-spectrum antiviral effects through viral RNA binding and degradation」主題。共同形成了獨特的指紋。

引用此