MCF-10A-NeoST: A new cell system for studying cell-ECM and cell-cell interactions in breast cancer

Nicole Dodge Zantek, Jennifer Walker-Daniels, Jane Stewart, Rhonda K. Hansen, Daniel Robinson, Hui Miao, Bingcheng Wang, Hsing Jien Kung, Mina J. Bissell, Michael S. Kinch

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60 引文 斯高帕斯(Scopus)

摘要

Purpose: There is a continuing need for genetically matched cell systems to model cellular behaviors that are frequently observed in aggressive breast cancers. Experimental Design: We report here the isolation and initial characterization of a spontaneously arising variant of MCF-10A cells, NeoST, which provides a new model to study cell adhesion and signal transduction in breast cancer. Results: NeoST cells recapitulate important biological and biochemical features of metastatic breast cancer, including anchorage-independent growth, invasiveness in three-dimensional reconstituted membranes, loss of E-cadherin expression, and increased tyrosine kinase activity. A comprehensive analysis of tyrosine kinase expression revealed overexpression or functional activation of the Axl, FAK, and EphA2 tyrosine kinases in transformed MCF-10A cells. Conclusions: MCF-10A and these new derivatives provide a genetically matched model to study defects in cell adhesion and signaling that are relevant to cellular behaviors that often typify aggressive breast cancer cells.
原文英語
頁(從 - 到)3640-3648
頁數9
期刊Clinical Cancer Research
7
發行號11
出版狀態已發佈 - 1月 1 2001
對外發佈

ASJC Scopus subject areas

  • 腫瘤科
  • 癌症研究

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