Major vault protein regulates cell growth/survival signaling through oxidative modifications

Dividutta Das, Yi Hsuan Wang, Cheng Ying Hsieh, Yuichiro J. Suzuki

研究成果: 雜誌貢獻文章

7 引文 (Scopus)

摘要

Major vault protein forms a hollow, barrel-like structure in the cell called the vault, whose functions and regulation are not well understood. The present study reports that major vault protein regulates growth/survival signaling in human airway smooth muscle cells through oxidative modifications. The promotion of protein S-glutathionylation by asthma mediators such as interleukin-22 and platelet-derived growth factor or by knocking down glutaredoxin-1 or thioredoxin activated cell growth signaling. Mass spectrometry identified that major vault protein is glutathionylated. Major vault protein knockdown enhanced cell death and inhibited STAT3 and Akt signaling. We identified a protein partner of major vault protein that is regulated by glutaredoxin-1, namely myosin-9, which was found to serve as a cell death factor. Knocking down myosin-9 or promoting protein S-glutathionylation by knocking down glutaredoxin-1 inhibited the death of airway smooth muscle cells by heating to simulate bronchial thermoplasty, a clinically successful procedure for the treatment of severe asthma. These results establish a novel signaling pathway in which ligand/receptor-mediated oxidation promotes the S-glutathionylation of major vault protein, which in turn binds to myosin-9 to suppress the heating-induced death of airway smooth muscle cells.

原文英語
頁(從 - 到)12-18
頁數7
期刊Cellular Signalling
28
發行號1
DOIs
出版狀態已發佈 - 一月 1 2016
對外發佈Yes

指紋

Cell Survival
Glutaredoxins
Myosins
Growth
Smooth Muscle Myocytes
Protein S
Heating
Cell Death
Asthma
Thioredoxins
Platelet-Derived Growth Factor
major vault protein
Mass Spectrometry
Ligands
Survival
Proteins

ASJC Scopus subject areas

  • Cell Biology

引用此文

Major vault protein regulates cell growth/survival signaling through oxidative modifications. / Das, Dividutta; Wang, Yi Hsuan; Hsieh, Cheng Ying; Suzuki, Yuichiro J.

於: Cellular Signalling, 卷 28, 編號 1, 01.01.2016, p. 12-18.

研究成果: 雜誌貢獻文章

Das, Dividutta ; Wang, Yi Hsuan ; Hsieh, Cheng Ying ; Suzuki, Yuichiro J. / Major vault protein regulates cell growth/survival signaling through oxidative modifications. 於: Cellular Signalling. 2016 ; 卷 28, 編號 1. 頁 12-18.
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