Magnolol stimulates lipolysis in lipid-laden RAW 264.7 macrophages

Jin Shan Chen, Yuh Lien Chen, Andrew S. Greenberg, Yi Jen Chen, Seu Mei Wang

研究成果: 雜誌貢獻文章

23 引文 斯高帕斯(Scopus)

摘要

This study investigated the effect of magnolol, a compound isolated from Magnolia officinalis, on lipolysis in lipid-laden RAW 264.7 macrophages. Treatment of macrophages with magnolol led to dissolution of lipid droplets. This phenomenon was accompanied by a dose-dependent release of glycerol and cholesterol and a concomitant reduction in intracellular levels of glycerol and cholesterol. Furthermore, adipose differentiation-related protein (ADRP), a lipid droplet-associated protein, was down-regulated by magnolol in a dose- and time-dependent manner by Western blot analysis. Immunofluorescence studies also showed that ADRP became detached from the surface of lipid droplets after magnolol treatment. The lipolytic effect of magnolol was not mediated through the cAMP-protein kinase A (PKA) system, an authentic lipolytic pathway for macrophages, since magnolol did not induce an increase of intracellular cAMP levels, and pretreatment with either of PKA inhibitors, PKI and KT5720, did not abrogate the lipolytic response to magnolol. We conclude that magnolol induce-lipolysis of lipid-laden macrophages by down-regulation of ADRP expression and detachment of ADRP from the lipid droplet surface by a cAMP-independent mechanism. Lipolysis of lipid-laden macrophages may occur when the amount of ADRP on the surface of lipid droplets is not enough to stabilize the lipid droplets.
原文英語
頁(從 - 到)1028-1037
頁數10
期刊Journal of Cellular Biochemistry
94
發行號5
DOIs
出版狀態已發佈 - 四月 1 2005

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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    Chen, J. S., Chen, Y. L., Greenberg, A. S., Chen, Y. J., & Wang, S. M. (2005). Magnolol stimulates lipolysis in lipid-laden RAW 264.7 macrophages. Journal of Cellular Biochemistry, 94(5), 1028-1037. https://doi.org/10.1002/jcb.20361