Lysyl oxidase and enhancement of cell proliferation and angiogenesis in oral squamous cell carcinoma

Yin Hua Shih, Kuo Wei Chang, Michael Yuanchien Chen, Cheng Chia Yu, Dan Jae Lin, Shih Min Hsia, Heng Li Huang, Tzong Ming Shieh

研究成果: 雜誌貢獻文章

26 引文 斯高帕斯(Scopus)

摘要

Background Lysyl oxidase (LOX) is a copper-dependent enzyme that cross-links collagen and elastin in the extracellular matrix. LOX overexpressed in various tumors. The manner in which LOX affects tumor growth remains controversial. Methods Chemical treatment and gene transfection were used to induce LOX overexpression or inhibition in cell lines SAS and SVEC4-10. LOX mRNA, protein, and activity were confirmed before tube formation assay and tumorigenesis. The microvessels in the tumor section were detected by immunostaining CD31-positive endothelial cells. Results LOX overexpression and copper induction of LOX activity increased SVEC4-10 tube formation. LOX silencing and β-aminopropionitrile inhibition of LOX activity had opposite effects. LOX overexpression increased proliferation and proliferating cell nuclear antigen expression. High LOX expression clones increased tumor size in a tumorigenesis model. The microvascular numbers were higher in LOX overexpression tumors than in control tumors. Conclusion LOX can induce cell proliferation and angiogenesis in oral squamous cell carcinoma.

原文英語
頁(從 - 到)250-256
頁數7
期刊Head and Neck
35
發行號2
DOIs
出版狀態已發佈 - 二月 2013
對外發佈Yes

ASJC Scopus subject areas

  • Otorhinolaryngology

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