PROBLEM: Previously, we observed that follicular fluid obtained from patients with premature luteinization contained elevated interleukin-1β (IL-1β) levels. In this study, we aimed to examine the effects of luteinizing hormone (LH) on IL-1β expression and IL-1β on steroidogenesis in human granulosa-luteal cells. METHOD OF STUDY: Human granulosa-luteal cells were obtained during oocyte retrieval. The cells were treated with either LH or IL-1β and subsequently were examined for the level of IL-1β transcript. The conditioned media were examined for IL-1β protein and steroid hormone levels. RESULTS: LH (250-500 mIU/mL) up-regulated the expression of IL-1β mRNA (up to a 4-fold increase over control: P < 0.05) in the granulosa-luteal cells. IL-1β (5-50 ng/mL) increased the basal, but not LH-dependent, progesterone production from these cells in a dose-dependent manner after 96 and 144 hr of culture (P ( 0.05). However, an inhibitory effect of IL-IP on LH-dependent estradiol production was observed (up to 20% decrease, P < 0.05). CONCLUSIONS: LH is capable of stimulating IL-1β transcript expression in human granulosa-luteal cells and may regulate ovarian steroidogenesis, at least partly through the activation of IL-1β.
|頁（從 - 到）||125-133|
|期刊||American Journal of Reproductive Immunology|
|出版狀態||已發佈 - 三月 1 2000|
ASJC Scopus subject areas
- Obstetrics and Gynaecology
Chen, H. F., Shew, J. Y., Chao, K. H., Chang, L. J., Ho, H. N., & Yang, Y. S. (2000). Luteinizing hormone up-regulates the expression of interleukin-lβ mRNA in human granulosa-luteal cells. American Journal of Reproductive Immunology, 43(3), 125-133.