Low levels of IgG recognizing the α-1-Antitrypsin peptide and its association with taiwanese women with primary sjögren’s syndrome

Yu Sheng Chang, Chih Hong Pan, Che Chang Chang, Kai Leun Tsai, Han Wen Chou, Jin Hua Chen, Sheng Hong Lin, Yi Ying Lu, Chih Chun Tai, Yi Fang Lin, Ching Yu Lin

研究成果: 雜誌貢獻文章

1 引文 (Scopus)

摘要

The aim of this study was to examine oxidative stress and low level of α-1-antitrypsin (A1AT) in primary Sjögren’s syndrome (pSS), and evaluate the associated autoreactivity against unmodified and their 4-hydroxy-2-nonenal (HNE)-modified peptides with pSS. Two differentially expressed proteins, α-1-acid glycoprotein 1 (A1AG1) and A1AT, exhibited 2-fold differences, and their HNE modifications were identified by depleted-albumin and immunoglobulin G (IgG) serum protein, in-solution digestion, in-gel digestion, and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) from pSS patients and age-matched healthy controls (HCs). Furthermore, levels of proteins, confirmation of HNE modifications, HNE-protein adducts and autoreactivity against unmodified and their HNE-modified peptides were further validated. Levels of the HNE-protein adduct and A1AG1 were significantly higher in pSS patients than HCs, but levels of A1AT were significantly lower in pSS patients compared to HCs. Only the HNE modification of A1AT was confirmed. Our study suggests that elevated HNE-protein adduct, oxidative stress, level (odds ratio (OR) 4.877, p = 0.003), lowered A1AT level (OR 3.910, p = 0.010) and a decreased level of anti-A1AT50-63 IgG (OR 3.360, p = 0.010) showed an increased risk in pSS patients compared to HCs, respectively.
原文英語
文章編號2750
期刊International Journal of Molecular Sciences
18
發行號12
DOIs
出版狀態已發佈 - 十二月 18 2017

指紋

Peptides
peptides
Immunoglobulin G
Association reactions
Proteins
proteins
Oxidative stress
adducts
Odds Ratio
Digestion
Oxidative Stress
Glycoproteins
Level control
Liquid chromatography
liquid chromatography
Mass spectrometry
Tandem Mass Spectrometry
2-nonenal
albumins
Liquid Chromatography

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

引用此文

@article{443989d5914649ec87e1108069915c2c,
title = "Low levels of IgG recognizing the α-1-Antitrypsin peptide and its association with taiwanese women with primary sj{\"o}gren’s syndrome",
abstract = "The aim of this study was to examine oxidative stress and low level of α-1-antitrypsin (A1AT) in primary Sj{\"o}gren’s syndrome (pSS), and evaluate the associated autoreactivity against unmodified and their 4-hydroxy-2-nonenal (HNE)-modified peptides with pSS. Two differentially expressed proteins, α-1-acid glycoprotein 1 (A1AG1) and A1AT, exhibited 2-fold differences, and their HNE modifications were identified by depleted-albumin and immunoglobulin G (IgG) serum protein, in-solution digestion, in-gel digestion, and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) from pSS patients and age-matched healthy controls (HCs). Furthermore, levels of proteins, confirmation of HNE modifications, HNE-protein adducts and autoreactivity against unmodified and their HNE-modified peptides were further validated. Levels of the HNE-protein adduct and A1AG1 were significantly higher in pSS patients than HCs, but levels of A1AT were significantly lower in pSS patients compared to HCs. Only the HNE modification of A1AT was confirmed. Our study suggests that elevated HNE-protein adduct, oxidative stress, level (odds ratio (OR) 4.877, p = 0.003), lowered A1AT level (OR 3.910, p = 0.010) and a decreased level of anti-A1AT50-63 IgG (OR 3.360, p = 0.010) showed an increased risk in pSS patients compared to HCs, respectively.",
keywords = "4-hydroxy-2-nonenal, Autoantibody isotypes, Inhibitor, Primary Sj{\"o}gren’s syndrome, Serum, α-1-antitrypsin",
author = "Chang, {Yu Sheng} and Pan, {Chih Hong} and Chang, {Che Chang} and Tsai, {Kai Leun} and Chou, {Han Wen} and Chen, {Jin Hua} and Lin, {Sheng Hong} and Lu, {Yi Ying} and Tai, {Chih Chun} and Lin, {Yi Fang} and Lin, {Ching Yu}",
year = "2017",
month = "12",
day = "18",
doi = "10.3390/ijms18122750",
language = "English",
volume = "18",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "MDPI AG",
number = "12",

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TY - JOUR

T1 - Low levels of IgG recognizing the α-1-Antitrypsin peptide and its association with taiwanese women with primary sjögren’s syndrome

AU - Chang, Yu Sheng

AU - Pan, Chih Hong

AU - Chang, Che Chang

AU - Tsai, Kai Leun

AU - Chou, Han Wen

AU - Chen, Jin Hua

AU - Lin, Sheng Hong

AU - Lu, Yi Ying

AU - Tai, Chih Chun

AU - Lin, Yi Fang

AU - Lin, Ching Yu

PY - 2017/12/18

Y1 - 2017/12/18

N2 - The aim of this study was to examine oxidative stress and low level of α-1-antitrypsin (A1AT) in primary Sjögren’s syndrome (pSS), and evaluate the associated autoreactivity against unmodified and their 4-hydroxy-2-nonenal (HNE)-modified peptides with pSS. Two differentially expressed proteins, α-1-acid glycoprotein 1 (A1AG1) and A1AT, exhibited 2-fold differences, and their HNE modifications were identified by depleted-albumin and immunoglobulin G (IgG) serum protein, in-solution digestion, in-gel digestion, and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) from pSS patients and age-matched healthy controls (HCs). Furthermore, levels of proteins, confirmation of HNE modifications, HNE-protein adducts and autoreactivity against unmodified and their HNE-modified peptides were further validated. Levels of the HNE-protein adduct and A1AG1 were significantly higher in pSS patients than HCs, but levels of A1AT were significantly lower in pSS patients compared to HCs. Only the HNE modification of A1AT was confirmed. Our study suggests that elevated HNE-protein adduct, oxidative stress, level (odds ratio (OR) 4.877, p = 0.003), lowered A1AT level (OR 3.910, p = 0.010) and a decreased level of anti-A1AT50-63 IgG (OR 3.360, p = 0.010) showed an increased risk in pSS patients compared to HCs, respectively.

AB - The aim of this study was to examine oxidative stress and low level of α-1-antitrypsin (A1AT) in primary Sjögren’s syndrome (pSS), and evaluate the associated autoreactivity against unmodified and their 4-hydroxy-2-nonenal (HNE)-modified peptides with pSS. Two differentially expressed proteins, α-1-acid glycoprotein 1 (A1AG1) and A1AT, exhibited 2-fold differences, and their HNE modifications were identified by depleted-albumin and immunoglobulin G (IgG) serum protein, in-solution digestion, in-gel digestion, and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) from pSS patients and age-matched healthy controls (HCs). Furthermore, levels of proteins, confirmation of HNE modifications, HNE-protein adducts and autoreactivity against unmodified and their HNE-modified peptides were further validated. Levels of the HNE-protein adduct and A1AG1 were significantly higher in pSS patients than HCs, but levels of A1AT were significantly lower in pSS patients compared to HCs. Only the HNE modification of A1AT was confirmed. Our study suggests that elevated HNE-protein adduct, oxidative stress, level (odds ratio (OR) 4.877, p = 0.003), lowered A1AT level (OR 3.910, p = 0.010) and a decreased level of anti-A1AT50-63 IgG (OR 3.360, p = 0.010) showed an increased risk in pSS patients compared to HCs, respectively.

KW - 4-hydroxy-2-nonenal

KW - Autoantibody isotypes

KW - Inhibitor

KW - Primary Sjögren’s syndrome

KW - Serum

KW - α-1-antitrypsin

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U2 - 10.3390/ijms18122750

DO - 10.3390/ijms18122750

M3 - Article

AN - SCOPUS:85038428385

VL - 18

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

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M1 - 2750

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